软坚消瘿颗粒对肝郁脾虚型桥本甲状腺炎大鼠甲状腺细胞凋亡的影响  

Effects of Ruanjian Xiaoying Granules on thyroid cell apoptosis in Hashimoto thyroiditis rats due to Liver Depression and Spleen Deficiency Pattern

作　　者：李叙颖 张兰[2] 王琳[1] 武佳琦 范思有 刘佳[1] LI Xu-ying;ZHANG Lan;WANG Lin;WU Jia-qi;FAN Si-you;LIU Jia(Liaoning University of Traditional Chinese Medicine,Shenyang 110847,China;The Hospital Affiliated to Liaoning University of Traditional Chinese Medicine,Shenyang 110032,China)

机构地区：[1]辽宁中医药大学,辽宁沈阳110847 [2]辽宁中医药大学附属医院,辽宁沈阳110032

出　　处：《中成药》2019年第4期774-778,共5页Chinese Traditional Patent Medicine

基　　金：2016辽宁省科学技术基金项目(201602505)

摘　　要：目的考察软坚消瘿颗粒对肝郁脾虚型桥本甲状腺炎大鼠甲状腺细胞凋亡的影响。方法高碘饮水联合皮下注射甲状腺球蛋白建立桥本甲状腺炎大鼠模型,慢性束缚应激、过度疲劳、饮食失节等复合方法建立肝郁脾虚大鼠模型。36只造模大鼠随机分为模型组、雷公藤多苷片组、软坚消瘿颗粒组,另取12只正常大鼠作为正常组,给药8周后采集大鼠腹主动脉血和甲状腺组织,ELISA法检测血清TGAb、TPOAb水平,TUNEL法检测甲状腺细胞凋亡数,Western blot法检测甲状腺组织TLR2、TLR4、MyD88、NF-κB p65蛋白表达。结果与模型组比较,软坚消瘿颗粒组TGAb、TPOAb水平,TLR2、TLR4、MyD88、NF-κB p65蛋白表达显著降低(P<0.01),细胞凋亡数显著减少(P<0.01)。结论软坚消瘿颗粒可抑制肝郁脾虚型桥本甲状腺炎大鼠甲状腺细胞凋亡,其机制可能与抑制TLRs/MyD88/NF-κB信号通路有关。AIM To investigate the effects of Ruanjian Xiaoying Granules on thyroid cell apoptosis in Hashimoto thyroiditis rats due to Liver Depression and Spleen Deficiency Pattern.METHODS The rat models for Hashimoto thyroiditis and Liver Depression and Spleen Deficiency were established by high iodine drinking water combined with hypodermic injection of thyroglobulin and composite methods(chronic restraint stress,excessive fatigue,dietetic dissection,etc.),respectively.Thirty-six modeled rats were randomly divided into model group,Tripterygium Glycosides Tablets group and Ruanjian Xiaoying Granules group,and another 12 normal rats were recruited into normal group.After 8-week administration,rat abdominal aorta blood and thyroid tissue were collected,TGAb,TPOAb levels in serum were detected by ELISA,thyroid cell apoptosis count was detected by TUNEL,TLR2,TLR4,MyD88,NF-κB p65 protein expressions in thyroid tissue were detected by Western blot.RESULTS Compared with the model group,the Ruanjian Xiaoying Granules group demonstrated significantly decreased TGAb,TPOAb levels and TLR2,TLR4,MyD88,NF-κB p65 protein expressions(P<0.01),markedly reduced cell apoptosis count(P<0.01).CONCLUSION Ruanjian Xiaoying Granules can inhibit thyroid cell apoptosis in Hashimoto thyroiditis rats due to Liver Depression and Spleen Deficiency Pattern,whose mechanism may contribute to the inhibition of TLRs/MyD88/NF-κB signaling pathway.

关 键 词：软坚消瘿颗粒 桥本甲状腺炎 肝郁脾虚 甲状腺细胞 凋亡 

分 类 号：R285.5[医药卫生—中药学]