新型吡咯并嘧啶衍生物N67对3T3-L1细胞的抑制作用及机制初探  

Inhibitory effect and mechanism of a novel pyrrolopyrimidine derivative N67 on 3T3-L1 cells

作　　者：易晓红[1] 李汶峰 钟占琼 贺玉萍[2] 谢璐霜[1] 陈继兰 李敏[1] 龚圆渊[1] 杨岚[1] Yi Xiaohong;Li Wenfeng;Zhong Zhanqiong(Dept of Embyology,Chengdu University of Traditional Chinese Medicine,Chengdu 611137;Dept of Science Pf Prescription,Chengdu University of Traditional Chinese Medicine,Chengdu 611137;Experimental Science Center,Chengdu University of Traditional Chinese Medicine,Chengdu 611137)

机构地区：[1]成都中医药大学基础医学院组胚教研室,成都611137 [2]成都中医药大学基础医学院方剂学教研室,成都611137 [3]成都中医药大学基础医学院实验科研中心,成都611137

出　　处：《安徽医科大学学报》2019年第3期380-384,共5页Acta Universitatis Medicinalis Anhui

基　　金：四川省教育厅自然基金(编号:16ZB0124);成都中医药大学自然基金(编号:ZRMS201232)

摘　　要：目的探讨新型吡咯类小分子化合物N67对小鼠前脂肪细胞3T3-L1的作用效果和机制。方法培养Swiss小鼠3T3-L1细胞,设对照组,将N67以不同时间的浓度作用于3T3-L1细胞,采用MTT细胞毒性检测,求出IC_(50);流式细胞术检测细胞凋亡情况;Western blot检测相关蛋白表达。结果 N67对3T3-L1细胞的生长有显著抑制作用,流式细胞术显示能诱导其凋亡,且有时间和浓度依赖性。Western blot结果证明其可能通过ABHD6靶点发挥作用。结论新型小分子化合物N67可诱导3T3-L1凋亡,作用机制可能是通过抑制ABHD6表达,证明N67有可能作为新的ABHD6抑制剂。Objective To explore the pharmacological action and it’s mechanism of a new small molecule compounds N67 on the Swiss 3 T3-L1 preadipocytes.Methods 3 T3-L1 cells were cultured,and control group and match group were divided.MTT assay was used to detect cell viability,flow cytometry was used to detect apoptosis,and Western blot was used to detect protein expression.Results The N67 inhibited 3 T3-L1 cells’proliferation with time and dose dependent manner.N67 induced apoptosis of 3 T3-L1 cells and decreased the expression of ABHD6.Conclusion Inconsiderationof ABHD6 is one of the potential targets for treatment of nonalcoholic fatty liver disease.The results proved that N67 could be used as one of the ABHD6 inhibitor,meanwhile,N67 may serve as a new rapeutic agent of fatty transformation diseases.

关 键 词：新型小分子化合物N67 前脂肪细胞3T3-L1 凋亡 ABHD6 抑制剂 

分 类 号：R961[医药卫生—药理学]