姜黄素对胃癌细胞增殖、迁移及Hsp90/JAK/STAT3通路的影响  

Effect of curcumin on the proliferation,migration and Hsp90/JAK/STAT3 pathway of gastric carcinoma cells

作　　者：李磊 陈志武 Li Lei;Chen Zhiwu(Dept of Pharmacology,Basic Medical College of Anhui Medical University,Hefei 230032)

机构地区：[1]安徽医科大学基础医学院药理学教研室,合肥230032

出　　处：《安徽医科大学学报》2019年第3期448-451,457,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金项目(编号:81374002)

摘　　要：目的探讨姜黄素对胃癌细胞增殖与侵袭迁移的影响及其相关作用机制,为胃癌诊断、治疗提供新的途径。方法用CCK8法比较不同处理组胃癌细胞的增殖能力,同时用Trans-well和划痕试验检测药物处理后细胞侵袭与迁移情况,通过实时荧光定量PCR、Western blot等技术检测癌细胞增殖相关信号通路热休克蛋白90-贾纳斯激酶/信号转导因子和转录激活因子3(Hsp90-JAK/STAT3)中关键基因的转录与翻译水平。结果 CCK8试验结果显示,姜黄素能明显抑制胃癌SGC-7901细胞株的增殖活性(P<0.01);Transwell和划痕实验检测显示姜黄素对胃癌细胞的侵袭迁移能力起到显著抑制作用(P<0.01);实时荧光定量PCR、Western blot检测结果显示,姜黄素处理可使胃癌细胞中Hsp90、JAK2和STAT3的基因转录、蛋白表达水平降低(P<0.01)。上述指标均呈现一定的浓度依赖性。结论姜黄素能够显著降低胃癌细胞SGC-7901的增殖能力并抑制其侵袭和迁移,而这一过程可能与姜黄素对胃癌细胞中Hsp90-JAK/STAT3信号通路激活的抑制有关。因此,姜黄素在胃癌治疗方面可能会发挥重要作用。Objective To investigate the effect of curcumin on the proliferation and invasion/migration of gastric carcinoma cells and its potential action mechanism,and to provide a new way for the treatment of gastric cancer.Methods CCK8 proliferation assay was conducted to compare the proliferation of SGC-7901 cell incurcumin treatment groups and control group.Wound healing assay and in vitro invasion assay were used to investigate theinvasion and migration activities of gastric carcinoma cells after the administration of curcumin.The gene transcription and protein expression of Hsp90,JAK2,and STAT3 were determined by RT-qPCR and Western blot.Results Curcumin resulted in significant decrease in proliferation of SGC-7901 cells(P<0.01).The invasion and migration of tested cells was also significantly inhibited(P<0.01)by the curcumin treatment.Moreover,the Hsp90 expression,the activation JAK2 and STAT3 were decreased(P<0.01)by the curcumin administration.All tested indexes showed obviously dose-dependent.Conclusion Curcumin could significantly reduce the proliferation ability of gastric cancer cell and inhibit its invasion and migration,which might be related to its inhibition of the activation of Hsp90-JAK/STAT3 signaling pathway.Curcumin may play an important role in the treatment of gastric cancer.

关 键 词：姜黄素 胃癌细胞 增殖 侵袭迁移 Hsp90-JAK/STAT3 

分 类 号：R965.1[医药卫生—药理学]