儿童急性淋巴细胞白血病中SOCS3通过STAT3信号通路调节Treg细胞表达  

SOCS3 regulates Treg cell expression via STAT3 signaling pathway in childhood with acute lymphoblastic leukemia

作　　者：黄玲玲[1] 王宁玲[1] 吴正玉 刘亢亢[1] 储金华[1] 杨林海[1] Huang Lingling;Wang Ningling;Wu Zhengyu(Dept of Pediatrics,The Second Affiliated Hospital of Anhui Medical University,Hefei 230601)

机构地区：[1]安徽医科大学第二附属医院儿科,合肥230601

出　　处：《安徽医科大学学报》2019年第3期458-461,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽高校省级自然科学研究项目(编号:KJ2012A168)

摘　　要：目的探讨儿童急性淋巴细胞白血病(ALL)细胞因子信号转导抑制因子-3 (SOCS3)的表达水平对调节性T细胞(Treg细胞)表达水平的影响。方法选取45例初治的ALL患儿作为初诊组,13例健康儿童作为正常对照组。采用荧光定量聚合酶链式反应(PCR)法对实验对象外周血单个核细胞中信号转导与转录激活子-3(STAT3)mRNA、SOCS3 mRNA的表达水平进行测定,采用流式细胞术对Treg细胞的表达水平进行测定。结果①ALL患儿初诊组在SOCS3 mRNA表达水平明显低于正常对照组(P<0.05);而STAT3 mRNA表达水平明显高于正常对照组(P<0.05);②ALL患儿初诊组Treg细胞的表达水平高于正常对照组(P<0.05);③ALL患儿初诊组中STAT3的表达水平与Treg细胞的表达水平呈正相关(r=0.751,P<0.05),SOCS3的表达水平与STAT3、Treg细胞的表达水平均呈负相关(STAT:r=-0.61,P<0.05;Treg:r=-0.558,P<0.05)。结论 ALL患儿初诊组中SOCS3的低表达引起STAT3的活化增加,进而提高了Treg细胞的表达水平,通过调控SOCS3表达水平的方法可能为ALL的肿瘤免疫治疗提供新的研究思路。Objective To investigate the role of suppressor of cytokine signaling 3(SOCS3)in the expression of Treg cells in children with acute lymphoblastic leukemia(ALL).Methods Forty-five children with ALL and thirteen age-matched healthy children were selected,then the expression levels of SOCS3 mRNA,signal transducers and activators of transcription 3(STAT3)mRNA were measured by real-time polymerase chain reaction(RT-PCR).Meanwhile,the proportion of CD4^+CD25^+FOXP3^+regulatory Treg cells was analyzed by flow cytometry.Results①Compared with normal control group,the expression level of SOCS3 in ALL patients was obviously weakened(P<0.05),while that of STAT3 in ALL patients was significantly elevated(P<0.05).②The proportion of Treg cells significantly increased in ALL compared with normal control group.③A positive correlation(r=0.751,P<0.05)between STAT3 expression levels and proportion of Treg cells was observed in ALL patients,while the expression level of SOCS3 in ALL patients was negatively correlated with that of STAT3 and Treg cells(r=-0.61,P<0.05).Conclusion Silencing of SOCS3 in pediatric ALL increases the expression of Treg cells by activating STAT3 signaling pathway,which may provide a new way for immune therapy via improving the expression level of SOCS3 in pediatric ALL patients.

关 键 词：急性淋巴细胞白血病 细胞因子信号转导抑制因子-3 调节性T细胞 信号转导与转录激活子-3 

分 类 号：R725.5[医药卫生—儿科]