咽喉宁口服液抗菌、镇痛、止痒作用及对急性细菌性咽炎的治疗作用研究  

Pharmacological Research of Yanhouning Oral Liquid on Antibiosis, Analgesia, and Relieve Itching and Its Therapeutic Effects on Acute Bacterial Pharyngitis

作　　者：孙静[1] 陆赛卫 高英杰[1] 赵荣华[1] 郭姗姗[1] 时宇静[1] 曲天歌 玄振玉[2] 崔晓兰[1] SUN Jing;LU Saiwei;GAO Yingjie;ZHAO Ronghua;GUO Shanshan;SHI Yujing;QU Tiange;XUAN Zhenyu;CUI Xiaolan(Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;Suzhou Youseen New Drug R&D Co. Ltd.,Suzhou 215001,China;Dongzhimen Hospital of Beijing University of Chinese Medicine,Beijing 100700,China)

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]苏州玉森新药开发有限公司,江苏苏州215001 [3]北京中医药大学东直门医院,北京100700

出　　处：《中医药学报》2019年第2期14-18,共5页Acta Chinese Medicine and Pharmacology

基　　金：国家自然科学基金项目(81774204)

摘　　要：目的:研究咽喉宁口服液的抗菌、镇痛、止痒作用及对乙型溶血性链球菌感染致大鼠急性咽炎模型的治疗作用。方法:采用乙型溶血性链球菌咽部感染致大鼠急性咽炎模型评价其对大鼠咽部病变的治疗作用,二倍稀释法评价其体外抗菌作用,金黄色葡萄球菌感染致小鼠死亡模型评价其体内抗菌作用,热板致小鼠疼痛模型评价其镇痛作用,磷酸组织胺致豚鼠瘙痒模型评价其止痒作用。结果:咽喉宁口服液1.16 g干膏粉/(kg·d)、0.58 g干膏粉/(kg·d)、0.29 g干膏粉/(kg·d)3个剂量组均能降低大鼠咽部病变积分,提高小鼠镇痛率及豚鼠致痒阈,与模型对照组比较有显著性差异(P<0.05、P<0.01)。体外抑菌实验结果显示咽喉宁口服液对所试6种病原菌均具有不同程度的抑制作用,体内抑菌实验结果显示咽喉宁口服液中剂量组能明显降低金黄色葡萄球菌感染小鼠的死亡率、延长小鼠平均存活天数,与模型对照组比较有显著性差异(P<0.05、P<0.01)。结论:咽喉宁口服液具有抗菌、镇痛、止痒作用,能减轻乙型溶血性链球菌所致大鼠急性咽炎咽部病变。Objective: To evaluate the effects of antibiosis, analgesia, and relieving itching of Yanhouning Oral Liquid(YHNOL), and to observe its therapeutic effect on acute bacterial pharyngitis. Methods: Pharyngitis model was established by injecting of beta hemolytic streptococcus into pharyngeal mucosa of rats. The in vitro antibacterial effect was observed by double dilution method. The in vivo antibacterial effect was observed by death model of staphylococcus aureus infection in mice. The analgesic effect was investigated by hot plate method. The antipruritic effect was investigated by phosphoric acid histamine induced itching experiment in guinea pigs. Results: Three doses(1.16 g dry extract (kg·d)^-1 , 0.58 g dry extract (kg·d)^-1 , 0.29 g dry extract (kg·d)^-1 ) could significantly decreased pharyngeal lesions and increased the thresholds of pain and itch( P <0.05, P <0.01). YHNOL had different degrees of inhibitory effect on six kinds of bacteria in vitro. It could obviously reduce the death rate of staphylococcus aureus infected mice and extend the average survival days of mice in vivo( P <0.05, P <0.01). Conclusion: YHNOL had significant effect of antibiosis, analgesia, and relieving itching. It also could effectively alleviate the symptoms of acute pharyngitis induced by staphylococcus aureus in rats.

关 键 词：咽喉宁口服液 急性细菌性咽炎 抗菌 镇痛 止痒 

分 类 号：R285.5[医药卫生—中药学]