MicroRNA-122-5p与MicroRNA-199a-5p在EMS患者血清的表达及临床意义  

Expression and clicnial significance of miR-122-5p and miR-199a-5p in serum of patients with endometriosis

作　　者：袁琳[1] 张建洁[1] 陈雪[1] 郑艳[1] 姚燕 李平[2] Lin Yuan;Jian-jie Zhang;Xue Chen;Yan Zheng;Yan Yao;Ping Li(Department of Gynaecology,North China Petroleum Administration Bureau General Hospital,Renqiu,Hebei 062552,China;Department of Otorhinolaryngology,North China Petroleum Administration Bureau General Hospital,Renqiu,Hebei 062552,China)

机构地区：[1]华北石油管理局总医院妇科,河北任丘062552 [2]华北石油管理局总医院耳鼻喉科,河北任丘062552

出　　处：《中国现代医学杂志》2019年第7期48-52,共5页China Journal of Modern Medicine

摘　　要：目的探讨micro RNA-122-5p(mi R-122-5p)与micro RNA-199a-5p(mi R-199a-5p)在子宫内膜异位症(EMS)患者血清的表达及临床意义。方法前瞻性设计,随机选取2016年1月—2017年12月华北石油管理局总医院诊治的EMS患者90例(EMS组)及同期不孕症检查的患者70例(对照组)为研究对象。分别采用实时荧光定量聚合酶链反应(qRT-PCR)及酶联免疫吸附试验(ELISA)检测两组人群血清的mi R-122-5p和mi R-199a-5p的表达及白细胞介素-6(IL-6)的表达,并分析其与临床特征的相关性。构建受试者操作特征(ROC)曲线。结果 EMS组患者的血清及腹腔液中IL-6、miR-122-5p、miR-199a-5p的表达与对照组比较,差异有统计学意义(P <0.05),EMS组均增高。miR-122-5p的表达与IL-6水平(r=0.578,P <0.05)、miR-199a-5p表达(r=0.721,P <0.05)呈正相关。miR-199a-5p的表达与IL-6水平(r=0.562,P<0.05)呈正相关。miR-122-5p和miR-199a-5p检测EMS的敏感性分别为94.7%和92.1%,特异性分别为90.5%和88.6%。结论血清miR-122-5p和miR-199a-5p在EMS患者中的表达增加,miR-122-5p与miR-199a-5p有成为诊断子宫内膜异位症的生物标志物的潜力。Objective To explore the expression and clinical significance of miR-122-5p and miR-199a-5p in patients with endometriosis(EMS).Methods Ninety patients with endometriosis and patients receiving infertility examination were involved in this study.Quantitative RT-PCR and enzyme linked immunosorbent assay were performed to detect the expression levels of miR-122-5p,miR-199a-5p and interleukin-6.Clinical relationships of the mentioned index with clinical features were analyzed.Results The serum levels of IL-6,miR-122-5p,and miR-199a-5p were significantly higher in endometriosis patients than in control group(P<0.05).Expression of miR-122-5p was positively correlated with serum IL-6(r=0.578)and miR-199a-5p(r=0.721);miR-199a-5p expression was positively correlated with serum IL-6(r=0.562).Sensitivity of circulating miR-122-5p and miR-199a-5p was 94.7%and 92.1%,respectively.Specificity of circulating miR-122-5p and miR-199a-5p was 90.5%and 88.6%,respectively.Conclusions Serum miR-122-5p and miR-199a-5p may serve as diagnostic biomarkers for endometriosis.

关 键 词：子宫内膜异位症 miR-122-5p/microRNAs miR-199a-5p/microRNAs 

分 类 号：R713[医药卫生—妇产科学]