玻璃化冷冻对体外受精-胚胎移植出生子代胎盘印记基因Snrpn表达的影响  

Effect of vitrification on the expression of imprinted gene Snrpn of neonatal placental tissues

作　　者：姚建凤 黄燕芳[2] 黄荣富[3] 林素霞 郭彩琼 吴培雅 李友筑[4] 胡继芬[2] Yao Jianfeng;Huang Yanfang;Huang Rongfu;Lin Suxia;Guo Caiqiong;Wu Peiya;Li Youzhu;Hu Jifeng(Reproductive Medicine Center,Quanzhou Maternity & Child Healthcare Hospital,Quanzhou 362000,China;Health Checkup Center,The First Affiliated Hospital,Fujian Medical University,Fuzhou 350004,China;Clinical Testing Center,The Second Affiliated Hospital,Fujian Medical University,Quanzhou 362000,China;Reproductive Medicine Center,The First Affiliated Hospital,Xiamen University,Xiamen 361002,China)

机构地区：[1]泉州市妇幼保健院生殖医学中心,362000 [2]福建医科大学附属第一医院健康体检中心,福州350004 [3]福建医科大学附属第二医院检验科,泉州362000 [4]厦门大学附属第一医院生殖医学中心,361002

出　　处：《中华生殖与避孕杂志》2019年第2期97-102,共6页Chinese Journal of Reproduction and Contraception

基　　金：泉州市科技局2016年度科技计划项目(2016Z38);泉州市科技项目B类课题([2004]0010).

摘　　要：目的探讨玻璃化冷冻技术对出生子代胎盘组织印记基因Snrpn表达的影响。方法收集2015年3月1日—2017年10月31日期间在泉州市妇幼保健院生殖医学中心接受体外受精-胚胎移植(IVF-ET)治疗的不孕患者分娩后的胎盘组织,按移植胚胎是否冻融分为新鲜周期组和玻璃化冷冻组;收集同期正常妊娠妇女分娩后的胎盘组织作为对照组。运用Real-time RT-PCR和Western blotting分别检测胎盘组织中印记基因 Snrpn表达水平。结果与正常对照组相比,新鲜周期组和玻璃化冷冻组胎盘组织Snrpn mRNA水平和SNRPN蛋白质水平均表达升高,差异有统计学意义(mRNA:P均<0.001;蛋白质:P=0.008,P=0.005),但新鲜周期组和玻璃化冷冻组组间差异均无统计学意义(P=0.212,P=0.286)。结论IVF一定程度上影响了胎盘组织Snrpn基因的表达,而玻璃化冷冻技术本身可能并不加重或减轻这种影响。Objective To investigate the effect of vitrification on the expression of imprinted geneSnrpn of neonatal placental tissues. Methods The neonatal placental tissue after delivery from infertility patients undergoingin vitro fertilization-embryo transfer (IVF-ET) treatment at the Reproductive Medicine Center of Quanzhou Maternal and Child Health Hospital during the period from 1 March 2015 to 31 October 2017, were collected respectively. Samples were divided into fresh group and vitrified group according to the pregnancy outcomes following fresh or vitrified embryo transfer. The placenta tissue from the natural pregnancy was collected as control group. Real-time RT-PCR and Western blotting were used to detect theSnrpn mRNA levels and SNRPN protein levels in placenta tissues from the above three groups. Results Compared with control group, the expression ofSnrpn mRNA and SNRPN protein in the placenta from the fresh group and the vitrified group increased, with a statistically significant difference (mRNA: allP<0.001;protein:P=0.008,P=0.005). There was no significant difference between the fresh group and the vitrified group (P=0.212,P=0.286). Conclusion IVF may affect the imprinting geneSnrpn transcriptional expression in mRNA and protein levels to some extent, and the vitrification technology may not increase or reduce this impact.

关 键 词：受精 体外 胚胎移植 玻璃化冷冻 Snrpn基因 

分 类 号：R714.8[医药卫生—妇产科学]