IL-10通过抑制IFN-γ和IL-2表达促进鼠衣原体感染  

IL-10 promotes Chlamydia muridarum infection in mice through inhibiting IFN-γ and IL-2 expression

作　　者：贺庆芝 贺梦婷 王昕 罗晓清 吴移谋 钟光明[2] He Qingzhi;He Mengting;Wang Xin;Luo Xiaoqing;Wu Yimou;Zhong Guangming(Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study Center,University of South China,Hengyang 421001,China;The University of Texas Health Science Center at San Antonio,San Antonio 78229,USA)

机构地区：[1]南华大学药学与生物科学学院,湖南省分子靶标新药研究协同创新中心,衡阳421001 [2]美国德克萨斯大学圣安东尼奥健康科学中心,圣安东尼奥78229

出　　处：《中华微生物学和免疫学杂志》2019年第2期126-130,共5页Chinese Journal of Microbiology and Immunology

基　　金：湖南省自然科学基金(2O18JJ2326);国家自然科学基金(81202323).

摘　　要：目的初步探讨IL-10促进鼠衣原体感染的可能分子机制。方法鼠衣原体经灌胃或子宫内感染C57BL/6野生型和IL-10^-/-小鼠后,采用间接免疫荧光试验检测肠道和生殖道内鼠衣原体的生长情况;输卵管积水评分评估生殖道疾病严重程度;酶联免疫吸附试验(ELISA)检测血液中IFN-γ和IL-2的表达水平。结果与野生型小鼠相比,IL-10^-/-小鼠的肠道和生殖道均具有较强的衣原体清除能力,同时细胞因子IFN-γ和IL-2的表达显著性增加。此外,野生型小鼠表现出更严重的输卵管病理损伤。结论IL-10通过抑制IFN-γ和IL-2的表达使宿主清除衣原体能力下降,从而促进衣原体在小鼠体内生长,加重输卵管积水的发病率。Objective To study the possible molecular mechanism of IL-10 in promoting Chlamydia muridarum infection in mice.Methods C57BL/6 wild-type and IL-10 gene knockout(IL-10^-/-)mice were infected with Chlamydia muridarum.Indirect immunofluorescence assay was used to detect the growth of Chlamydia muridarum in the intestinal and genital tracts.The severity of genital diseases was assessed by hydrosalpinx scoring.Expression of IFN-γ and IL-2 in blood was measured by ELISA.Results Compared with the wild-type group,Chlamydia clearance in the intestinal and genital tracts of IL-10^-/- mice was significantly faster,and the expression of IFN-γ and IL-2 increased significantly.In addition,wild-type mice showed more serious hydrosalpinx.Conclusions IL-10 delays Chlamydia trachomatis clearance and promotes Chlamydia infection through inhibiting the expression of IFN-γ and IL-2,which aggravates hydrosalpinx.

关 键 词：沙眼衣原体 1L-10 细胞因子 免疫 

分 类 号：R392[医药卫生—免疫学]