乙型肝炎病毒B和C基因型S蛋白特异性CTL表位保守性分析  

Conservative analysis in CTL epitopes of HBV S protein between B and C genotypes

作　　者：陈庆新 李文姝[1] 陈卓艳 何佳莉 汪文寰 林茂 叶菊秀 CHEN Qingxin;LI Wenshu;Chen Zhuoyan;HE Jiali;Wang Wenhuan;Lin Mao;YE Juxiu(Institute of Microbiology and Immunology,Wenzhou Medical University,Wenzhou 325035,China;School of Ophthalmology & Optometry,Wenzhou Medical University,Wenzhou 325035,China)

机构地区：[1]温州医科大学微生物学与免疫学教研室,浙江温州325035 [2]温州医科大学眼视光学院,浙江温州325035

出　　处：《温州医科大学学报》2019年第4期253-257,共5页Journal of Wenzhou Medical University

基　　金：浙江省自然科学基金资助项目(LY13C010003);温州市科技计划项目(Y20080008);温州医科大学人才启动项目(QTJ07003)

摘　　要：目的:分析国内流行乙型肝炎病毒(HBV)基因型S区基因编码蛋白的特异性CTL表位保守性,发现稳定且特异性的CTL表位。方法:设计HBV S基因特异性引物,克隆S基因并构建到pIRES载体;利用3种表位分析方法对HBV B和C基因型S区基因编码蛋白特异性CTL表位进行分析筛选,对候选CTL表位用BLAST搜索HBV蛋白库,分析候选CTL表位在HBV B和C基因型中的保守性。结果:成功克隆HBV S基因并构建真核表达载体;根据设定条件,利用生物信息学分析方法对S区基因编码蛋白进行分析共筛选到17个CTL表位,其中已经鉴定6个,未鉴定表位11个;保守性分析发现,在B和C基因型中具有较高保守性的有6个(>80%),均是未鉴定的表位,其余表位(包括6个已鉴定)的保守性较低。结论:国内流行HBV基因型间S区基因编码蛋白的特异性CTL表位存在较大差异性,共筛选到6个保守性较高的特异性CTL表位可用于进一步的免疫学研究。Objective:To analyze the conservative property in CTL epitopes of HBV large S proteins and figure out the stable epitopes between B and C genotypes.Methods:A pair of primers for S gene of HBV was designed,the S gene amplified by PCR and construed into pIRES vector.According to large S protein from B and C genotypes of HBV,specific CTL epitopes in large S protein of HBV had been analyzed and screened by BIMAS,NetMHC and SYFPEITHI.Conservative property analysis was made by comparing candidate CTL epitopes with large S protein database of HBV B and C genotypes conducted by BLAST.Results:HBV S gene was cloned into pIRES expression vector successfully.Seventeen CTL epitopes of large S protein were screened according to scores of BIMAS,NetMHC or SYFPEITHI.High conservative property in B and C genotypes of HBV(>80%)was showed in 6 CTL epitopes which were not identified now.17 CTL epitopes,including 6 identified CTL epitopes,were not conservative in B and C genotypes of HBV.Conclusion:The specific CTL epitopes of large S protein of HBV show high differences between B and C genotypes.Six CTL epitopes of HBV large S protein are obtained for further immunological studies.

关 键 词：HBV 基因型 CTL表位 保守性分析 

分 类 号：R373.2[医药卫生—病原生物学]