4种切梢小蠹携带伴生菌的带菌率差异  

Carrying Rates and Differences of Fungi Associated with Four Tomicus Species in Yunnan

作　　者：王艺璇[1,2] 王晓渭 陈鹏 袁瑞玲[1,2] 冯丹 杜春花[1] 叶辉 潘悦[3] 吕军 WANG Yi-xuan;WANG Xiao-wei;CHEN Peng;YUAN Rui-ling;FENG Dan;DU Chun-hua;YE Hui;PAN Yue;LV Jun(Forest Protection Institute, Yunnan Academy of Forestry,Kunming 650201,Yunnan,China;School of Agriculture, Yunnan University,Kunming 650091,Yunnan,China;Yunnan Biotechnology Research Center for Prevention and Control Technology of Crop Pest,Kunming 650091,Yunnan,China)

机构地区：[1]云南省林业科学院,云南昆明650201 [2]云南省作物病虫害生物防控技术研究中心,云南昆明650091 [3]云南大学农学院,云南昆明650091

出　　处：《林业科学研究》2019年第2期53-59,共7页Forest Research

基　　金：云南省应用基础重点项目:"云南省切梢小蠹伴生真菌种类及在小蠹蛀害中的协同互作机制"(2013FA055);云南省青年项目:"伴生真菌与切梢小蠹聚集行为关系初探"(2017FD171)

摘　　要：[目的]为了弄清中国云南4种切梢小蠹伴生菌的携带率差异,了解伴生菌在蠹害过程中的互作关系。[方法]采用人工接种法和野外调查法,检测松树韧质部是否蓝变,判断切梢小蠹带菌情况,统计分析小蠹试验材料的带菌率。[结果]云南切梢小蠹成虫带菌率(80%)明显高于横坑切梢小蠹(63%)、松芽小蠹(53%)、华山松切梢小蠹(56%)。切梢小蠹幼虫的带菌率最高(90%),其次是蛹的带菌率(78%),新成虫的带菌率最低(58%)。切梢小蠹头(33%)、足(30%)、翅(32%)3个重要器官带菌率差异不明显。林间调查表明,切梢小蠹伴生菌提高了小蠹蛀干繁殖的成功率。[结论]首次报道了中国云南4种切梢小蠹伴生菌的带菌特性及其差异,研究结果为揭示云南省切梢小蠹成灾机制及探索切梢小蠹有效防控新途径提供了科学数据支持。[Objective] The purpose of the study is to clarify the carrying rates and the differences of fungi associated with four Tomicus species in Yunnan, China and understand the relationships between the beetles and these fungi during Tomicus attacking process.[Method] Fungal carrying rate was analyzed by detecting stained phloem based on artificial inoculation and field survey.[Result] The fungal carrying rate of Tomicus yunnanensis (80%) was distinctly higher than that of the other three Tomicus species:T. minor (63%), T. armandii (56%), and T. brevipilosus (53%);the fungal carrying rate of Tomicus-larva (90%) was higher than its pupae (78%) and new adult (58%);No significant difference in fungal carrying rate was observed among heads (33%), wings (32%) and legs (30%) of Tomicus-adults. According to field survey, the associated fungi significantly improved the breeding success rate of Tomicus on the trunk.[Conclusion] This is the first report on the carrying rates and the differences of fungi associated with four Tomicus species in Yunnan, China. The results provide new data for reveal outbreak mechanism of Tomicus species in Yunnan, China.

关 键 词：切梢小蠹 伴生真菌 带菌率 危害程度 

分 类 号：S763.11[农业科学—森林保护学]