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作 者:于少帅[1] 赵文霞[1] 姚艳霞[1] 淮稳霞[1] 肖文发[1] YU Shao-shuai;ZHAO Wen-xia;YAO Yan-xia;HUAI Wen-xia;XIAO Wen-fa(Key Laboratory of Forest Protection of National Forestry and Grassland Administration, Research Institute of Forest Ecology Environment and Protection, Chinese Academy of Forestry,Beijing 100091,China)
机构地区:[1]中国林业科学研究院森林生态环境与保护研究所国家林业和草原局森林保护学重点实验室,北京100091
出 处:《林业科学研究》2019年第2期111-116,共6页Forest Research
基 金:国家重点研发计划项目(2016YFC0501503);中央级公益性科研院所基本科研业务费专项资金(CAFYBB2017SZ003)
摘 要:[目的]分析同一采样地、同一生境新疆野苹果枯枝症状级别与水杨酸含量、胸径的关系,寻找与枯死症状可能相关的特征。[方法]通过超声提取、HPLC检测分析不同枯枝症状级别样品中水杨酸组成和含量差异,并对枯枝症状级别与水杨酸含量、胸径关系进行统计分析。[结果]不同枯枝症状级别的新疆野苹果枝叶中水杨酸含量在39.5~122.6 mg·kg^(-1)之间,含量差异显著(P<0.05)。枯枝症状从0级到Ⅴ级,水杨酸含量存在先降低后升高的趋势。不同枯枝症状级别新疆野苹果胸径大小变异系数差异明显,Ⅴ级样品个体间胸径变异系数相对较大(39.18%);Ⅰ级样品变异系数相对较小(30.28%)。新疆野苹果水杨酸含量和胸径大小呈负相关(P>0.05),其中,0级、Ⅴ级样品水杨酸含量和胸径大小呈正相关,Ⅰ级、Ⅱ级、Ⅲ级、Ⅳ级样品水杨酸含量和胸径大小呈负相关(P>0.05)。[结论]新疆野苹果枯枝症状级别与水杨酸含量、胸径大小关系较为密切,研究结果对于天山野果林的生态保育与修复、苹果优质新品种的筛选与培育等具有一定的参考价值。[Objective] To analyze the relationships among deadwood symptom grades, the salicylic acid content and the DBH of Malus sieversii trees collected in same sampling site and habitat conditions was analyzed in order to find the features of M. sieversii that might be related to the symptoms of exsiccation.[Method] The difference in salicylic acid composition and content in different symptom grades of M. sieversii was investigated by ultrasonic extraction and HPLC methods. Statistical analysis was conducted to find the relationships among deadwood symptom grades, the salicylic acid content and DBH of M. Sieversii.[Result] The content of salicylic acid in M. sieversii branches and leaves with different deadwood symptom grades was in the range of 39.5~122.6 mg·kg^-1, showing significant difference (P<0.05). Along with deadwood symptoms from grade 0 to grade Ⅴ, the salicylic acid content fell down at first and then up. The variation coefficient of DBH size of M. sieversii was significantly different. The variation coefficient of DBH among grade Ⅴ samples was relatively high (39.18%), and the coefficient of grade Ⅰ samples was comparatively low (30.28%). There was a negative correlation between salicylic acid content and DBH of M. sieversii (P>0.05). There was a positive correlation between salicylic acid content and DBH of grade 0 and grade Ⅴ samples, and there was a negative correlation between salicylic acid content and DBH of samples of grades Ⅰ,Ⅱ,Ⅲ and Ⅳ(P>0.05).[Conclusion] There is a comparatively close relationship among deadwood symptom grades, the salicylic acid content and the DBH of M. sieversii. The results achieved in the study will provide some reference clues for ecological conservation and restoration of wild fruit forest in Tianshan Mountains and the screening and cultivation of high-quality varieties of cultivated apple.
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