尿液中的足细胞标志蛋白定量检测应用于糖尿病和糖尿病肾病诊断中的价值比较  

A Comparison of Quantitative Detection of Podocalyxin in Urine for the Diagnosis of Diabetes Mellitus and Diabetic Nephropathy

作　　者：林兵英 罗晓燕 方周宾[2] 段纯 刘影林 LIN Bing-ying;LUO Xiao-yan;FANG Zhou-bin;DUAN Chun;LIU Ying-lin(Department of Clinical Laboratory,Affiliated Shenzhen Maternity & Child Healthcare Hospital,Southern Medical University,Shenzhen 518028,China;Department of Clinical Laboratory,Shenzhen Second People’s Hospital,Shenzhen 518035,China)

机构地区：[1]南方医科大学附属深圳妇幼保健院,广东深圳518028 [2]深圳市第二人民医院检验科,广东深圳518035

出　　处：《标记免疫分析与临床》2019年第4期604-606,共3页Labeled Immunoassays and Clinical Medicine

摘　　要：目的探讨糖尿病(DM)和糖尿病肾病(DN)患者尿液中足细胞标志蛋白(podocalyxin,PCX)定量检测的价值,为临床应用提供参考依据。方法选取我院2017年1月至2018年1月所收治糖尿病肾病患者60例为研究组,另选同期60例单纯糖尿病患者为对照组。采集两组患者血样及尿样,除检测PCX外,并检测其他肾功能相关指标,比较两组患者差异。结果两组患者在血清肌酐(SCr)、血清尿素氮(SBUN)指标方面差异无统计学意义(P>0. 05);研究组患者中,SCysC为1.37±0.19mg/L、Uα1-MG为30.12±8.55mg/L、URBP为85.72±10.29mg/L、UmALB/UCr为40.19±4.02mg/gCr、PCX为31.26±15.17ng/mL,均明显高于对照组,t分别为18.879、21.653、25.250、39.935、13.767,差异具有统计学意义(P<0.05)。两组患者在SCr、SBUN、URBP检出异常率方面无显著差异(P>0.05);研究组患者中,SCysC异常检出率为61.67%、Uα1-MG为58.33%、UmALB/UCr为58. 33%、PCX为71. 67%,与对照组相比较,差异具有统计学意义(P<0.05);其中,以PCX指标相差最为显著。结论 PCX应用于糖尿病肾小球早期损害鉴别诊断中,取材方便、异常检出率高,具有较高鉴别诊断价值,可视为一项理想检查指标。但PCX检测极易受运动、饮水等因素干扰,在实际鉴别诊断过程中,应加以规避。Objective To explore the clinical value of quantitative detection of podocyte marker protein (PCX) in urine of patients with diabetes mellitus (DM) and diabetic nephropathy (DN).Methods Sixty patients with diabetic nephropathy admitted to our hospital from January,2017 to January,2018 were selected as the study group,and another 60 patients with diabetes mellitus were selected as the control group.The blood and urine samples of the two groups were collected.In addition to PCX,other renal function related indexes were also detected,and the differences between the two groups were compared.Results There were no significant differences in serum creatinine (SCr) and serum urea nitrogen (SBUN) between the two groups (P >0.05).In the study group,SCysC was 1.37±0.19mg/L,along with U alpha 1-MG 30.12±8.55 mg/L,URBP 85.72±10.29 mg/L,UmALB/UCr 40.19±4.02 mg/gCr,and PCX 31.26±15.17 ng/mL,respectively,which were all higher than the control group.The t test results were 18.879,21.653,25.250,39.935 and 13.767,respectively,showed significances compared with the control group (P <0.05).There were no significant differences in the abnormal rates of SCysC,SBUN and URBP between the two groups(P >0.05).In the study group,the abnormal rate of SCysC was 61.67%,along with U alpha 1-MG 58.33%,UmALB/UCr 58.33%,and PCX 71.67%,all showed significances compared with the control group (P <0.05).Among them,PCX was the most significant one.Conclusion PCX can be used in the differential diagnosis of diabetic glomerular early lesion.It is convenient to obtain specimen and has a high detection rate of abnormalities.It has a high value in differential diagnosis and can be regarded as an ideal examination index.But PCX detection is very susceptible to exercise,drinking water and other factors.In the actual differential diagnosis process,these factors should be avoided.

关 键 词：PCX 糖尿病 糖尿病肾病 

分 类 号：R587.2[医药卫生—内分泌] R692.9[医药卫生—内科学]