嫁接对网纹甜瓜幼苗叶片氮代谢及转录因子CmHY5表达的影响  

Effects of Grafting on Nitrogen Metabolism and CmHY5 Gene Expression of Muskmelon Seedling Leaves

作　　者：王志冉 王红艳 张海旺 张颖 张海兵 康倩 陈丽娟 许传强[1] WANG Zhi-ran;WANG Hong-yan;ZHANG Hai-wang;ZHANG Ying;ZHANG Hai-bing;KANG Qian;CHEN Li-juan;XU Chuan-qiang(College of Horticulture/Key Laboratory of Protected Horticulture,Ministry of Education/Collaborative Innovation Center of Protected Vegetable Surround Bohai Gulf Region/ National & Local Joint Engineering Research Center of Northern HorticulturalFacilities Design & Application Technology,Shenyang Agricultural University,Shenyang 110161,China)

机构地区：[1]沈阳农业大学园艺学院/设施园艺省部共建教育部重点实验室/环渤海湾地区设施园艺蔬菜优质高效生产协同创新中心/北方园艺设施设计与应用技术国家地方联合工程研究中心,沈阳110161

出　　处：《沈阳农业大学学报》2019年第2期146-153,共8页Journal of Shenyang Agricultural University

基　　金：国家自然科学基金青年基金项目(31401917);辽宁省农业领域青年科技创新人才培养资助计划项目(2014050);辽宁省教育厅一般项目(L2014266);现代农业产业技术体系项目(CARS-25);沈阳市科技计划项目(17-147-1-00)

摘　　要：嫁接技术在甜瓜生产上的应用在一定程度上缓解了施肥和产量之间的矛盾,并可有效地防治土传病害,缓解土壤连作障碍。以白籽南瓜圣砧1号嫁接的网纹甜瓜幼苗为处理,以自根网纹甜瓜幼苗为对照,测定接穗生物量、氮素含量、氮代谢关键酶活性、氮代谢关键基因及其转运蛋白相关基因表达变化,并对氮代谢和转运蛋白相关基因上游的调控因子CmHY5转录因子时空表达特性进行分析,以期进一步探析嫁接影响网纹甜瓜幼苗叶片氮素代谢的生理和分子机制。试验结果表明:嫁接能够显著提高接穗生物量和叶片中NO3--N和NH4+-N含量。嫁接网纹甜瓜幼苗叶片氮代谢关键酶NR与GS的酶活性及其相关基因CmNR和CmGS相对表达量显著增加,硝酸转运蛋白基因NRT(CmNRT2.4、CmNRT2.5)和铵盐转运蛋白基因AMT(CmAMT2、CmAMT3)相对表达量显著上调。自然光照条件下,嫁接处理显著促进CmHY5表达,其相对表达量为对照的2.68倍;但在弱光和黑暗条件下,嫁接处理与对照间无显著差异。嫁接处理能够显著提高CmHY5在网纹甜瓜幼苗根、茎、叶中表达,嫁接处理叶片中的CmHY5相对表达量是对照的5.98倍。可见,转录因子CmHY5在嫁接促进网纹甜瓜幼苗叶片氮素代谢及其转运过程中具有重要调控作用。Grafting technology applied in muskmelon production could not only alleviate contradictions between fertilization and yield as well as continuous cropping obstacle,but also prevent soil diseases.In this experiment,we used the treatment of muskmelon seedlings grafted with white-seed pumpkin 'Sheng-zhen No.1' and self-rooted muskmelon seedlings as control to measure the plants' scion biomass,nitrogen contents,key enzyme activities,relative genes expression of nitrogen metabolism and nitrogen translocator.We also analyzed the spatio-temporal expression of CmHY5,an upstream regulator of the relative genes involved in nitrogen metabolism and nitrogen translocator in order to further explore physiological and molecular mechanism of muskmelon seedling leaves by grafting.The results showed that grafting significantly increased the scions' biomass and contents of NO3^--N and NH4^+-N in seedling leaves.NR and GS activities,relative expression quantities of CmNR and CmGS in leaves of grafted muskmelon seedling significantly not only increased,but also CmNRT2.4,CmNRT2.5 of nitrate translocator and CmAMT2,CmAMT3 of ammonium translocator were up-regulated by grafting.Meanwhile,we found that grafting remarkably induced up-regulated expression of CmHY5 in muskmelon seedling leaves.Under natural lighting condition,the grafting treatment promoted CmHY5 expression which were 2.68 folds of that of control.Under weak light treatment and dark condition,there was no significant difference between grafting treatment and control.CmHY5 expression was increased in root,stem and leaf of muskmelon seedling under grafting treatment,for example,CmHY5 expression in leaf was 5.98 folds of that of the control.Therefore,CmHY5,a transcription factor,played a pivotal,regulatory role in the process of grafting to accelerate the nitrogen metabolism and transport.

关 键 词：网纹甜瓜 嫁接 氮代谢 CmHY5 

分 类 号：S652.4[农业科学—果树学]