微生物驱油过程中配气对菌群结构及驱油效果的影响  

Effect of Gas Distribution on Flora Structure and Oil Displacement in MEOR

作　　者：刘涛[1] 汪庐山[2] 胡婧[1] 巴燕[1] 刘方 曹嫣镔[1] LIU Tao;WANG Lushan;HU Jing;BA Yan;LIU Fang;CAO Yanbin(Research Institute of Petroleum Engineering,Shengli Oilfield Company,Sinopec,Dongying,Shandong 257000,P R of China;Shengli OilfieldCompany,Sinopec,Dongying,Shandong 257001,P R of China)

机构地区：[1]中国石化胜利油田分公司石油工程技术研究院,山东东营257000 [2]中国石化胜利油田分公司,山东东营257001

出　　处：《油田化学》2019年第1期143-146,190,共5页Oilfield Chemistry

基　　金：国家高技术研究发展计划(863计划)"中高温油藏微生物驱油关键技术研究"(项目编号2013AA064401)

摘　　要：为揭示油藏条件下微生物驱油过程中配注空气对菌群结构及驱油效果的影响规律,通过岩心驱替模拟实验研究了配气量对微生物驱驱油效率的影响,并通过显微镜、高通量测序仪和气相色谱分析了产出液中的菌群和小分子酸的动态变化。结果表明,微生物驱油过程中,配注空气量对菌群结构及驱油效果的影响显著。配注不同体积的空气激活微生物作用后产生了明显的驱替效果,并且随着配气量的增加驱替效率升高并逐渐稳定,液气比为1∶5时的驱替效率最高(8.77%)。随配气量增加产出液中菌数升高,不同类型的功能微生物被激活,其中4类主要优势功能菌占整个菌群比例的67%,功能微生物主要以产表面活性剂和乳化剂的芽孢杆菌和假单胞菌为主。配气量为液气比1∶5时微生物群落shannon多样性指数最高,代谢产生的小分子酸浓度较高,微生物代谢活性最佳。液气比1∶5可以作为现场实际配气的选择依据。In order to reveal the effect of air injection on flora structure and oil displacement efficiency during microbial flooding under reservoir condition,the effect of gas distribution on microbial flooding efficiency was studied by core displacement simulation experiment. The dynamic changes of microflora and small molecule acids in effluent were analyzed by microscopy,high throughput sequencer and gas chromatography. The results showed that air injection rate had a significant influence on flora structure and oil displacement effect in the process of microbial oil displacement. The obvious displacement effect was produced after the action of microbes. The displacement efficiency increased first and then stabilized with increasing gas distribution. When the ratio of liquid to gas was 1∶5,the displacement efficiency was the highest(8.77%). With the increase of gas distribution,the number of bacteria in the effluent increased,and different types of functional microorganisms were activated. Among them,four dominant functional bacteria accounted for 67% of the total bacterial population. The main functional microorganisms were Bacillus spp. and Pseudomonas spp. which produced surfactants and emulsifiers. The Shannon diversity index of the microbial community was the highest when the liquid gas ratio was 1∶5. Moreover,the higher concentration of small acid produced,the best microbial metabolic activity was. When the liquid gas ratio was 1∶5,it could be used as the basis for selecting actual gas distribution in the field.

关 键 词：微生物驱油 空气 菌群 小分子酸 驱油效果 

分 类 号：TE357[石油与天然气工程—油气田开发工程]