屠宰场猪肉中猪霍乱沙门氏杆菌的分离鉴定及16S rRNA的序列分析  

Isolation,identification and 16S rRNA sequence analysis of Salmonella choleraesuis from pork in slaughterhouses

作　　者：杨佰启 温贵兰[1,2] 邱燕 张升波 徐丽 李昌红[1,2] 林汉卿 田浪[1,2] YANG Baiqi;WEN Guilan;QIU Yan;ZHANG Shengbo;XU Li;LI Changhong;LIN Hanqing;TIAN Lang(College of Animal Science,Guizhou University,Guiyang 550025,China;Guizhou Provincial Key Laboratory of Animal Disease and Veterinary Public Health,Guiyang 550025,China;Anshun Animal Health Supervision Institute,Anshun 561000,China)

机构地区：[1]贵州大学动物科学学院,贵阳550025 [2]贵州省动物疫病与兽医公共卫生重点实验室,贵阳550025 [3]安顺市动物卫生监督所,贵州安顺561000

出　　处：《黑龙江畜牧兽医》2019年第6期73-77,171,共6页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(31460668);贵州省科学技术基金项目(黔科合J字[2015]2046号);贵州大学博士基金项目(贵大人基合字[2013]12号);贵州省高层次创新型人才培养项目(黔财教[2017]号);贵州省研究生工作站计划项目(GZZ2017002)

摘　　要：为了了解屠宰场生猪肉被沙门氏杆菌污染的情况,试验对采集的20份生猪肉进行细菌分离培养、选择性培养、生化试验、革兰氏染色、沙门氏杆菌外膜蛋白C(Omp C)基因PCR检测等方法进行鉴定,采用MegAlign软件绘制分离菌16S rRNA遗传进化树。结果表明:从屠宰场生猪肉中分离到一株疑似沙门氏杆菌,命名为GZ-Q1株; GZ-Q1株为革兰氏阴性的短小杆菌;生化试验结果与沙门氏杆菌的生化特性基本相符; Omp C基因PCR检测可见约204 bp的目的条带; GZ-Q1株与猪霍乱菌株(CP007639. 1)的同源性最高,为99. 9%。说明从屠宰场生猪肉中分离的GZ-Q1株为猪霍乱沙门氏杆菌。In order to understand the contamination of raw pork from slaughterhouses by Salmonella, 20 samples of raw pork were identified by bacterial isolation, selective culture, biochemical test, Gram staining, Salmonella outer membrane protein C(Omp C) PCR deteection, and 16S rRNA phylogenetic tree of isolated bacteria was drawn by MegAlign software. The results showed that a suspected Salmonella strain named GZ-Q1 was isolated from raw pork in slaughterhouses. GZ-Q1 strain was Gram-negative Bacillus pumilus. The results of biochemical tests were basically consistent with biochemical characteristics of Salmonella. Omp C PCR showed a target band of about 204 bp. GZ-Q1 strain had the highest homolog}-(99. 9%) with swine cholera strain (CP007639. 1). It indicates that the GZ-Q1 strain isolated from raw pork in slaughterhouses is Salmonella choleraesuis.

关 键 词：猪霍乱沙门氏杆菌 猪肉 分离鉴定 OMPC 16S RRNA 屠宰场 

分 类 号：S852.614[农业科学—基础兽医学] S851.43[农业科学—兽医学]