广西地区猪圆环病毒2型和猪圆环病毒3型基因型监测及遗传进化分析  

Genotype detection and phylogenetic analysis of Porcine circovirus 2 and Porcine circovirus 3 in Guangxi region

作　　者：李金凤 哈卓 辛佳亮[4] 李卓昕 张涵 郭影成[6] 郑敏 那少龙[1] 鲁会军 金宁一 LI Jinfeng;HA Zhuo;XIN Jialiang;LI Zhuoxin;ZHANG Han;GUO Yingcheng;ZHENG Min;NA Shaolong;LU Huijun;JIN Ningyi(Institute of Military Veterinary, Academy of Military Medicine, Academy of Military Sciences, Changchun 130122, China;College of Veterinary Medicine, Jilin University, Changchun 130062, China;College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China;College of Animal Science and Technology, Guangxi University, Nanning 530004, China;College of Agriculture, Yanbian University, Yanbian 133002, China;Jilin Fengman Area Animal Prevention and Control Center, Jilin 132013, China;Guangxi Center for Animal Disease Prevention and Control, Nanning 530001, China)

机构地区：[1]军事科学院军事医学研究院军事兽医研究所,长春130122 [2]吉林大学动物医学学院,长春130062 [3]东北农业大学动物医学学院,哈尔滨150030 [4]广西大学动物科学技术学院,南宁530004 [5]延边大学农学院,吉林延边133002 [6]吉林市丰满区动物疫病预防控制中心,吉林吉林132013 [7]广西动物疫病预防控制中心,南宁530001

出　　处：《黑龙江畜牧兽医》2019年第5期10-15,22,共7页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家重点研发计划项目(2017YFD0500101;2018YFD0500104)

摘　　要：为了明确猪圆环病毒2型(Porcine circovirus type 2,PCV-2)和猪圆环病毒3型(Porcine circovirus type 3,PCV-3)在广西地区的分子流行病学情况,试验通过PCR方法对2017年来自广西不同地区的80份猪肺脏样品进行PCV-2和PCV-3核酸检测,并对PCV-2和PCV-3的阳性样品进行ORF2基因的扩增和测序,同时利用生物信息学软件DNAStar和MEGA 6.06对PCV-2和PCV-3的ORF2基因进行同源性分析和构建遗传进化树。结果表明:2017年广西地区PCV-2和PCV-3的总感染率分别为43.8%(35/80)和33.8%(27/80),PCV-2和PCV-3的共感染率为16.3%(13/80)。试验获得的19个PCV-2 ORF2基因序列之间的核苷酸同源性为93.6%～99.9%,对应氨基酸同源性为93.1%～100%;19个PCV-2 ORF2基因序列与国内外参考毒株ORF2基因序列的核苷酸同源性为82.8%～99.9%,对应氨基酸同源性为83.3%～99.6%。试验获得的2个PCV-3 ORF2基因序列之间的核苷酸同源性为99.8%,对应氨基酸同源性为100%;2个PCV-3 ORF2基因序列与国内外参考毒株ORF2基因序列的核苷酸同源性为97.7%～99.8%,对应氨基酸同源性为96.2%～100%。获得的19个PCV-2 ORF-2基因序列中,12个为PCV-2b亚型,7个为PCV-2d亚型;获得的2个PCV-3 ORF2基因序列为PCV-3a亚型,与丹麦和中国湖南分离得到的PCV-3亲缘关系较近。说明PCV-2和PCV-3在广西地区猪群中感染率很高,PCV-2感染主要以PCV-2b亚型为主,PCV-3之间高度保守,分布无地域性规律。To understand the molecular epidemiology of PCV-2 and PCV-3 in Guangxi, a total of 80 lung samples from Guangxi in 2017 were detected for PCV-2 and PCV-3 by using PCR method. The positive samples of PCV-2 and PCV-3 were amplified and sequenced for ORF2 gene. Homology analysis and genetic tree construction of ORF2 genes of PCV-2 and PCV-3 were conducted using bioinformatics software DNAStar and MEGA 6. 06.The result showed that the total infection rate of PCV-2 and PCV-3 were 43. 8%(35/80) and 33. 8%(27/80) respectively ,co-infection rate of PCV-2 and PCV-3 was 16. 3%( 13/80). The nucleotide homology was 93. 6%-99. 9%, and the corresponding amino acid homology were 93. 1%- 100%, respectively. The nucleotide homology of ORF2 gene sequences of 19 ORF-2 strains between domestic and foreign reference strains B were 82. 8%-99. 9%, and the homology of corresponding amino acids were 83. 3%-99. 6%.The nucleotide homology between the ORF2 gene sequences of the two PCV-3 strains was 99. 8%, and the homology of the corresponding amino acids was 100%.The nucleotide homology between ORF2 gene sequence of two PCV-3 strains and ORF2 gene sequence of foreign reference strains were 97. 7%-99. 8%, and the homology of corresponding amino acids were 96. 2%-100%.Of the 19 PCV-2 ORF-2 gene sequences obtained, 12 were PCV-2b subtypes and 7 were PCV-2d subtypes. The two PCV-3 ORF-2 gene sequences obtained were PCV-3a subtypes, which were closely related to PCV-3 isolated from Denmark and Hunan, China. It indicated that PCV-2 and PCV-3 had a high infection rate in pigs farms in Guangxi. PCV-2 infection was mainly caused by PCV-2b subtype, and PCV-3 strains were highly conserved and distributed without regional rules.

关 键 词：猪圆环病毒2型 猪圆环病毒3型 ORF2基因 遗传进化分析 同源性分析 分子流行病学 

分 类 号：S852.659.2[农业科学—基础兽医学]