分子佐剂Ov-ASP-1对弓形虫SAG1核酸疫苗的影响  

Effect of molecular adjuvant Ov-ASP-1 on SAG1 nucleic acid vaccine of Toxoplasma gondii

作　　者：徐焘杰 李元凯 林霞琳 黄志坚 殷光文 XU Taojie;LI Yuankai;LIN Xialin;HUANG Zhijian;YIN Guangwen(College of Animal Science, Fujian Agriculture and Forestry University/Fujian Animal Medicine Engineering Laboratory, Fuzhou 350002, China)

机构地区：[1]福建农林大学动物科学学院/福建省动物药物工程实验室,福州350002

出　　处：《黑龙江畜牧兽医》2019年第5期118-122,共5页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金青年科学基金项目(31502058);福建农林大学临床兽医学教学团队建设项目(111416036);福建省高校杰出青年人才培育项目(KLa16029A);福建省高校新世纪优秀人才项目(KLa17059A)

摘　　要：为了研究分子佐剂Ov-ASP-1对弓形虫表面抗原糖蛋白1(SAG1)核酸疫苗的免疫效力的影响,用含有分子佐剂Ov-ASP-1的pVAX1-Ov-ASP-1-SAG1质粒、对照质粒pVAX1-SAG1、空载体pVAX1免疫鸡,并设置PBS对照组,通过抗体水平与细胞因子水平的检测结果判断免疫效果和免疫应答情况;然后用弓形虫RH株人工感染试验鸡,通过实时荧光定量PCR检测鸡的肺脏、脾脏、肝脏中的弓形虫数量,判断分子佐剂Ov-ASP-1是否增强鸡对弓形虫的抵抗力。结果表明:与其他组相比,试验各阶段pVAX1-Ov-ASP-1-SAG1组免疫球蛋白G(IgG)、白细胞介素-10(IL-10)、白细胞介素-12(IL-12)和干扰素γ(IFN-γ)水平都较高,差异达到了显著或极显著水平(P<0.05或P<0.01);攻虫后pVAX1-Ov-ASP-1-SAG1组感染弓形虫的鸡荷虫量比其他组极显著降低(P<0.01)。说明分子佐剂Ov-ASP-1可以显著增强弓形虫表面膜蛋白SAG1的免疫原性和免疫保护性。In order to study the effects of molecular adjuvant Ov-ASP-1 on the immune efficacy of SAG1 protein of Toxoplasma gondii ( T.gondii},chikens were divided into four groups ( pVAX1 -Ov - ASP - 1 -SAG1 group, pVAX1 -SAG1 group, pVAX1 group and PBS control group), then the antibody and cytokines levels were determined to indicate the immune effects and response. Then the chickens were artificially infected with T. gondii RH strain.Real-time fluorescence quantitative PCR was used to determine the number of toxoplasma in lung, spleen, and liver of chickens, to determine whether the molecular adjuvant-Ov-ASP-1 chould enhance the resistance of chickens to T. gondii. Results showed that antibody IgG level and cytokines IL-10, IL- 12, and IFN-γ were higher in the pVAX1-Ov-ASP1-SAG1 group than that in other groups( P<0. 05 or P<0. 01). The amount of parasites in chickens infected with T. gondii in pVAXl-Ov-ASP1-SAG1 group was extremly lower than that in other groups( P<0. 01). It indicates that the molecular adjuvant Ov-ASP - 1 can significantly enhance the immunogenicity and immunoprotection of SAG1 protein of T. gondii.

关 键 词：SAG1 蛋白 Ov-ASP-1 弓形虫 核酸疫苗 

分 类 号：S852.721[农业科学—基础兽医学] S852.5[农业科学—兽医学]