木薯种质'F200'耐盐生理特性探究  

Study on Salt-tolerant Physiological Characteristics of Cassava Germplasm 'F200'

作　　者：吕瑞 姚远[2] 段瑞军[2] 李瑞梅[2] 刘姣[2] 符少萍[2] 胡新文 郭建春[2] Lii Rui;Yao Yuan;Duan Ruijun;Li Ruimei;Liu Jiao;Fu Shaoping;Hu Xinwen;Guo Jianchun(College of Agriculture,Hainan University,Haikou,570228;Institute of Tropical Bioscience and Biotechnology,Chinese Academy of TropicalAgricultural Sciences,Haikou,571101)

机构地区：[1]海南大学热带农林学院,海口570228 [2]中国热带农业科学院热带生物技术研究所,海口571101

出　　处：《分子植物育种》2019年第6期2004-2010,共7页Molecular Plant Breeding

基　　金：木薯现代农业产业技术体系项目(CARS-11-HNGJC);国家自然科学基金项目(31671767; 31600196; 3160-1359);中国热带农业科学院基本科研业务费专项资金项目(1630052015014)共同资助

摘　　要：为研究木薯(Manihot esculenta Crantz)种质‘F200’的耐盐性及其在盐胁迫下生理特性的变化,本试验在室内用300 mL 300 mmol/L的Na Cl处理植株,对照用Hoagland营养液代替,分别测定处理前和处理3 d、6 d、9 d、12 d的H_2O_2、MDA、相对电导率、CAT、POD、SOD、脯氨酸、可溶性糖、相对含水量、叶绿素含量的变化。结果表明:在NaCl处理初期,H_2O_2含量增加,植株通过维持较高的SOD、POD、CAT等抗氧化酶活性,同时主动积累脯氨酸、可溶性糖等渗透调节物质来清除活性氧、维持细胞膜的稳定性及含水量,随着胁迫时间的增加,胁迫损伤逐渐加重,植株无法维持高水平的酶活性,酶活下降。因此,抗氧化酶活性的上升及渗透调节物质含量的增加可能是木薯种质‘F200’初期耐盐的主要生理因素,叶绿素含量则仅作为‘F200’耐盐性判断的参考指标,本研究为木薯耐盐机制的深入探究及其耐盐育种提供了参考依据。To study the salt tolerance of the cassava germplasm ’F200’(Manihot esculenta Crantz) and its physiological characteristics under salt stress, the plants were treated with 300 m L 300 mmol/L NaCl in the laboratory,the control was replaced with Hoagland nutrient solution. The changes of H2O2, MDA, relative conductivity, CAT,POD, SOD, proline, soluble sugar, relative water content and chlorophyll content were measured before and after treatment for 3 d, 6 d, 9 d and 12 d. The results showed that in the early stage of NaCl treatment, the content of H2O2 increased, and the plants maintained high antioxidant enzyme activities such as SOD, POD and CAT, and actively accumulated osmotic substances such as proline and soluble sugar to scavenge reactive oxygen species and maintain cell membrane. Stability and water content, with the increase of stress time, the stress damage gradually increased, the plant could not maintain high levels of enzyme activity, and the enzyme activity decreased. Therefore, the increase of antioxidant enzyme activity and osmotic adjustment substance content may be the main physiological factors of salt tolerance in cassava germplasm ’F200’ at the early stage, while chlorophyll content is only a reference index for judging salt tolerance of cassava germplasm ’F200’. All these provide a theoretical basis for further exploration of salt tolerance mechanism and salt tolerant breeding of cassava.

关 键 词：木薯 耐盐性 抗氧化酶 渗透调节 

分 类 号：S533[农业科学—作物学]