胆巴炮制对附子生物碱类成分的影响研究  

Study on the Effect of Brine Processing on Contents of Alkaloids in Aconiti Lateralis Radix Praeparata

作　　者：刘雨诗 刘红梅[1] 叶强[1] 方成鑫 刘晓梅 刘娟汝 郭力[1] LIU Yushi;LIU Hongmei;YE Qiang;FANG Chengxin;LIU Xiaomei;LIU Juanru;GUO Li(College of Pharmacy,Chengdu University of Traditional Chinese Medicine,The Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine,The State Key Laboratory of Resources Systems Research and Development Utilization of Traditional Chinese Medicine,Chengdu 611137 Sichuan,China)

机构地区：[1]成都中医药大学药学院教育部中药材标准化重点实验室中药资源系统研究与开发利用重点实验室,四川成都611137

出　　处：《中药新药与临床药理》2019年第4期472-477,共6页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家重点研发计划项目(2017YFC1701804);四川省青年科技创新研究项目(2017TD0001);四川省科技厅青年科技创新研究团队项目(2016TD0006)

摘　　要：目的比较有胆附片与无胆附片的成分异同,探讨胆巴炮制与无胆炮制对附子生物碱类成分的影响。方法采用酸性染料比色法及高效液相色谱法对5种附子炮制品(生附片、黑顺片、熟附片、蒸附片、炒附片)的生物碱类成分进行分析。结果江油产生附片质量最优,去皮可以提高附子中有效成分的相对含量;有胆炮制黑顺片、熟附片均能显著降低毒性成分双酯型生物碱的含量,但同时有效成分单酯型生物碱及总生物碱流失严重;无胆蒸制或炒制附片能有效去除毒性成分并保留有效成分,"去毒存性"能力较好。结论不同炮制方法能不同程度地降低附子毒性成分双酯型生物碱含量,增加有效成分单酯型生物碱含量,达到增效减毒的目的;但有胆炮制附片存在炮制过度之嫌,无胆炮制工艺简便易行,不易引入无机杂质,具有推广和应用价值。Objective To compare the differences between the brine processed and non-brine monkshood, and study the effects of different processing technology on the contents of alkaloids in Aconiti lateralis radix praeparata.Methods The alkaloids components of different products(Shengfupian-peeled, Heishunpian, Shufupian,Zhengfupian and Chaofupian) were analyzed by UV and HPLC method. Results The quality of the Jiangyou producing Shengfupian was better than the others,and the contents of the active components in aconite could be raised by peeling. Both Heishunpian and Shufupian pressed with brine can significantly reduce the contents of toxic component-the diester alkaloids,but the active ingredients,the monoester alkaloids and total alkaloids are greatly reduced. Non-brine Zhengfupian and Chaofupian can effectively remove the toxic components and retain the active ingredients. Conclusion Different processing technology can decrease the contents of diester alkaloids,increase the contents of monoester alkaloids,and achieve the purpose of increasing efficiency and reducing toxicity. However,the brine monkshood may be over-processed. The processing of non-brine monkshood is simple,without bringing in inorganic substances,which have promotional and application value.

关 键 词：附子 胆巴 炮制 生物碱 

分 类 号：R284.1[医药卫生—中药学]