我国不同地区小鼠Y染色体Zfy1基因遗传差异分析  

Genetic Difference Analysis of Zfy1 Gene on Y Chromosome of Mice in Different Regions of China

作　　者：陈梦璇 于立强 靖美东[1] CHEN Meng-xuan;YU Li-qiang;JING Mei-dong(School of Life Sciences,Ludong University,Yantai, Shandong264025;Yantai No. 3 Middle School, Yantai, Shandong264000)

机构地区：[1]鲁东大学生命科学学院,山东烟台264025 [2]山东省烟台第三中学,山东烟台264000

出　　处：《安徽农业科学》2019年第8期90-92,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(31371252)

摘　　要：[目的]对我国5个地区小鼠Zfy1基因进行遗传差异分析。[方法]通过对5个地区79个雄性小鼠样品进行DNA提取,PCR扩增后进行序列测定和分析。使用MEGA6.0和DNAsp等软件对获得序列进行分析,计算遗传距离,构建系统发育树。[结果]5个地区Zfy1基因碱基组成基本一致,碱基的平均含量为A 25.1%、T 39.6%、G 20.8%、C 14.4%,呈现出明显的AT偏向。79个样品共定义为13个单倍型,各单倍型间遗传距离小,无明显差异。以大鼠(GATN01000010)为外群,小鼠(NM_009570)为参照构建系统发育树,13个单倍型均处在同一分支上,无明显分化现象。[结论]我国南北方各地区间小鼠Zfy1基因无明显分化现象,基因差异性较小,具有极高的保守性。[Objective] To analyze the genetic difference of Zfy1 gene in 5 regions of China.[Method] DNA was extracted from 79 male mouse samples in 5 regions, and the sequence was determined and analyzed after PCR amplification. MEGA6.0, DNAsp and other software were used to analyze the sequences, and the genetic distance was calculated and the phylogenetic tree was constructed.[Result] The base pair composition was consistent among 5 regions. The average contentsof the bases were25.1% A, 39.6% T,20.8% G, and14.4% C, showing a significant AT bias. A total of 79 samples were defined as13 haplotypes, and there was no significant difference in genetic distance of each haplotype. The phylogenetic tree was constructed with rat GATN01000010 as the outgroup and mouse (NM_009570) as reference.13 haplotypes were clustered into the same branch, without obvious differentiation.[Conclusion] There was no obvious differentiation of Zfy1 gene in mice from north to south in China, and the genetic difference was small, Zfy1 gene had high conservation.

关 键 词：小鼠 Y染色体 Zfy1基因 遗传差异 

分 类 号：Q75[生物学—分子生物学]