桃拉病毒基因组的生物信息学分析  

Bioinformatics Analysis of Taura syndrome virus Genome

作　　者：李伟哲 贾阳阳 刘露[2] 肖勤[2] LI Wei-zhe;JIA Yang-yang;LIU Lu(College of Life Sciences,Hebei Agricultural University,Baoding,Hebei 071001;College of Ocean,Hebei Agricultural University,Qinhuangdao,Hebei 066003)

机构地区：[1]河北农业大学生命科学学院,河北保定071001 [2]河北农业大学海洋学院,河北秦皇岛066003

出　　处：《安徽农业科学》2019年第8期119-122,共4页Journal of Anhui Agricultural Sciences

基　　金：留学人员科技活动择优资助项目(CN201602)

摘　　要：[目的]对桃拉病毒(Taura syndrome virus,TSV)的完整基因组进行生物信息学分析。[方法]通过生物信息学方法对基因序列组成、开放阅读框、蛋白质理化性质、二级结构预测分析、蛋白跨膜结构的存在与否、蛋白信号肽存在与否以及蛋白质三级结构进行了预测分析。[结果]登录NCBI网站下载TSV(JX094350.1)10 128 bp的基因片段,经生物信息学分析,编码氨基酸3 286个,理论等电点(pI)为5.14,相对分子质量为366 443.00 Da,不稳定系数(Ⅱ)为37.76,属于稳定蛋白质;完整基因序列中包含2个开放阅读框(open reading frame,ORF);蛋白中存在跨膜结构;没有蛋白信号肽。[结论]对TSV的生物信息学分析有助于在分子水平上了解桃拉病毒的基因结构以及预测其感染机制,可为预防和治疗虾类的桃拉综合征提供有用的信息。[Objective]To make bioinformatics analysis on Taura syndrome virus (tsV) gene in swine.[Method]The complete genes of tsV were analyzed by bioinformatics software,including itsgene sequence analysis,open reading frame prediction(ORF) prediction,physicochemical properties of protein,secondary structure prediction,protein transmembrane and signal peptides prediction,and as well as protein tertiary structure prediction.[Result]The tsV gene (JX094350.1) with a length of10128 bp was successfully obtained from NCBI gene bank.The bioinformatics analysis showed that tsV gene was a total of 3286 amino acids,a theoretical isoelectric point (pI) of 5.14,a theoretical molecular mass of 366 443 Da,and an instability coefficient (Ⅱ) 37.76,being a stable protein.The complete gene sequence contained two open reading frames (ORFs).There was a transmembrane structure in the protein,and there was not included protein signal peptide.[Conclusion]The bioinformatics analysis of tsV is helpful for understanding Taura syndrome virus on molecular level and the prediction of infection mechanism.It will provide useful informations for the prevention and treatment of Taura syndrome.

关 键 词：桃拉病毒 基因 蛋白质 生物信息学分析 

分 类 号：S945.4[农业科学—水产养殖]