叶型对马尾松幼苗生长及叶绿素荧光特征的影响  

Effects of Leaf Types on Growth and Chlorophyll Fluorescence Characteristics in Pinus massoniana Seedlings

作　　者：王好运 吴峰 朱小坤[1] 谢维斌[3] Wang Haoyun;Wu Feng;Zhu Xiaokun;Xie Weibin(Institute for Forest Resources&Environment of Guizhou,Guizhou University Guiyang 550025;College of Forestry,Guizhou University Guiyang 550025;State Forest Farm in Duyun Duyun 558000)

机构地区：[1]贵州大学林学院,贵阳550025 [2]贵州大学贵州省森林资源与环境研究中心,贵阳550025 [3]贵州都匀国家马尾松良种基地,都匀558000

出　　处：《林业科学》2019年第3期183-192,共10页Scientia Silvae Sinicae

基　　金：国家自然科学基金项目(31660201);生态学一流学科建设项目(GNYL[2017]007);国家重点研发计划(2017YFD0600302)

摘　　要：【目的】研究叶型对马尾松幼苗生长及叶绿素荧光特征的影响,以期为马尾松苗木选择及栽培提供参考。【方法】以出圃时呈现为全初生条形叶、全次生针形叶和中间型的马尾松1年生幼苗为试验材料,分析其生长指标、光合色素含量及叶绿素荧光参数,研究初生叶和次生叶叶绿素荧光特征差异及对幼苗生长的影响。【结果】全次生叶幼苗出圃时苗木质量指数(QI)、干物质积累量及造林1年后净生长量均显著高于全初生叶幼苗;次生叶的叶长、体积和表面积等指标均显著大于初生叶;初生叶中叶绿素a、叶绿素b和总叶绿素含量均显著高于次生叶,两者间的类胡萝卜素含量无显著差异。初生叶的F_m、F_v/F_m、F_v/F_o均显著高于次生叶,F_o在2种叶型间无显著差异;初生叶的PSⅡ反应中心参数Y(Ⅱ)和F_v’/F_m’、淬灭参数q_P和NPQ、能量耗散参数Y(NPQ)和Y(NO)及光响应参数ETR_(max)、α、β和I_k也均高于次生叶,在高光强条件下,初生叶的非光化学淬灭和调节性能量耗散能力增强。由于次生针叶叶长、体积和表面积等形态指标均显著大于初生叶,因而具有次生针叶的幼苗的光合作用面积更大,干物质量积累较多,出圃时的苗木质量以及造林后的生长表现均高于全初生叶的幼苗。【结论】与次生叶相比,初生叶拥有较高的叶绿素含量,较耐光抑制,有较高的光能转化效率,但高光强条件下会通过热耗散的形式来减少多余光能对光合系统的损害;拥有次生叶的幼苗表现出较好的苗木质量及生长特性。【Objective】In this study, effects of leaf types on growth and chlorophyll fluorescence characteristicsin Pinus massoniana seedlings were studied in order to provide scientific basis for selection and cultivation.【Method】One-year-old seedlings of P.massoniana have three different leaf types, such as primary needles, needles and intermediate type, at nursery stage. In this experiment,the growth traits, photosynthetic pigment contents and chlorophyll fluorescence parameters were investigated on one-year-old seedlings of P.massoniana . The chlorophyll fluorescence characteristics of primary and secondary leaves and their effects on seedling growth were studied.【Result】The results showed thatthequality index (QI) at nursery stage, dry matter accumulationand growth traits one year after afforestation of the seedlings with needle swere significantly better than those with primary needles. The leaf length,volume and surface area of needles were significantly larger than the primary needles. Contents of chlorophyll a, chlorophyll b and total chlorophyll in primary needles were much higher than those in needles, while there was no significant difference in the content of carotenoid between the two types of needles. For chlorophyll fluorescence parameters, the F m , F v /F m and F v /F o in primary needles were significant higher than thosein needles.There was no significant difference in F o between the two leaf types.The Y (Ⅱ) and F v ′/F m ′ of PSⅡreaction center, q P and NPQ of quenching parameter, Y (NPQ) and Y (NO) of energy dissipation and the photoresponse parameters, such as ETR max ,α,β and I k in primary needles were significant higher than those in needles.It was indicated that the primary needles performed much better on the content of chlorophyll and light inhibition, with higher photosynthetic efficiency than needles, while the heat dissipation and non-photochemical quenching would increase under the intensivelight. However, due to larger leaf length, volume and surface area than primary

关 键 词：马尾松 初生条叶 次生针叶 叶绿素荧光 苗木质量 

分 类 号：S718.43[农业科学—林学]