西南地区HPV-6和HPV-11 E6/E7基因多态性分析  

Polymorphism Analysis of HPV-6 and HPV-11E6/E7 Genes in Southwest China

作　　者：万秋伶 丁显平[1] 高鹏 方莅媛 Wan Qiuling;Ding Xianping;Gao Peng;Fang Liyuan(Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education,Institute of Medical Genetics of College of Life Science of Sichuan University,Chengdu 610065,China;Bio-resource Research and Utilization Joint Key Laboratory of Sichuan and Chongqing,Sichuan 610064 & Chongqing 408400,China;Chongqing Innovation Academy of Characteristic Bioindustry Technology,Chongqing 408400,China)

机构地区：[1]四川大学生命科学学院生物资源与生态环境教育部重点实验室四川大学生命科学学院遗传医学研究所,成都610065 [2]特色生物资源资源研究与利用川渝共建重点实验室,四川610064重庆408400 [3]重庆特色生物产业技术创新研究院,重庆408400

出　　处：《成都医学院学报》2019年第2期153-157,共5页Journal of Chengdu Medical College

基　　金：四川省重点科研基金项目(No:2018JY0601)

摘　　要：目的研究西南地区人乳头瘤病毒6型(HPV-6)和11型(HPV-11)E6/E7基因多态性,为低危型HPV的防治提供分子水平。方法对HPV-6和HPV-11阳性标本扩增其目的基因,测序并对比Genenbank参考序列,对变异初步分析;PAML 4.8软件作用于E6/E7基因的选择压力。结果 HPV-6E6/E7基因共12个突变位点,其中有3个是首次发现的突变位点,5/12为非同义突变;HPV-11E6/E7中,共计5个单核苷酸突变,具有2/5非同义和3/5同义突变。选择压力结果显示,只有HPV-6E7基因有3个正向选择位点,分别是4R**,34E**和52F**。结论 HPV-6E6和E7序列在分析的群组中高度保守,表示HPV-6在进化的过程中,形成了有利于生存的分离株。HPV-11突变未发现正向选择位点,表明选择的变异对HPV-11来说适应环境较困难。Objective To investigate the polymorphism of E6/E7 genes of human papillomavirus type 6 (HPV-6) and human papillomavirus type 11 (HPV-11) in southwest China and provide the molecular level for the prevention and treatment of low-risk HPV. Methods The target genes from HPV-6 and HPV-11 positive samples was amplified, sequenced and compared with the Genenbank reference sequences for preliminary analysis of mutation. The software PAML 4.8 was used for the selection pressure of E6/E7 gene. Results There were 12 mutation sites in the HPV-6 E6/E7 gene, of which 3 were the mutation sites found for the first time and 5 were non-synonymous mutations. There were 5 single nucleotide mutations with 2 nonsynonymous mutations and 3 synonymous mutations in the HPV-11 E6/E7 gene. The results of selection pressure showed that the HPV-6 E7 gene had three positive selection sites which were 4R^**, 34E^** and 52F^**. Conclusion The HPV-6 E6/E7 sequences are highly conserved in the analyzed cohort, which indicates that HPV-6 forms survival-friendly isolates during the evolution process. No positive selection sites are found in the HPV-11 mutation, which indicates that the selected mutation is difficult to adapt to the environment for HPV-11.

关 键 词：人乳头瘤病毒6型 人乳头瘤病毒11型 基因多态性 低危型 选择压力 

分 类 号：Q754[生物学—分子生物学]