2018年河南省郑州市定点监测猪群伪狂犬病gE抗体检测  

Detection of gE Antibody against Pseudorabies in Swine Herds under Fixed-point Monitoring in Zhengzhou City of Henan Province in 2018

作　　者：贾松涛[1] 刘炜[1] 张军[1] 周婷婷[1] Jia Songtao;Liu Wei;Zhang Jun;Zhou Tingting(Zhengzhou Animal Disease Prevention and Control Center,Zhengzhou,Henan 450052,China)

机构地区：[1]郑州市动物疫病预防控制中心,河南郑州450052

出　　处：《中国动物检疫》2019年第4期5-8,共4页China Animal Health Inspection

摘　　要：为了解河南省郑州市2018年规模猪场的伪狂犬病野毒感染情况,利用gE抗体ELSIA方法,对免疫过猪伪狂犬病gE基因缺失疫苗的36个不同养殖规模的定点监测猪场,进行gE抗体检测。2018年1—12月,共检测血清样品1 057份,检出阳性场27个,场群阳性率为75.0%(27/36);检出阳性样品309份,样品阳性率为29.2%(309/1 057)。结果表明,郑州市伪狂犬病野毒感染面较广,流行率较高,存在较高的疫情暴发风险。存栏500头以下规模猪场的gE抗体阳性率(69.8%)是存栏3 000头以上的规模场(19.1%)的3.65倍,表明养殖规模越小,伪狂犬病野毒感染风险越大。利用荧光PCR方法对gE抗体阳性样品进行检测,检出阳性32份,阳性检出率为35.6%(32/90),表明感染猪群的持续带毒、排毒现象较为严重。建议规模化养猪场加强gE基因缺失疫苗免疫和gE抗体检测,及时淘汰阳性猪,同时结合隔离、消毒等综合防控技术,逐步实现猪伪狂犬病的控制与净化。In order to recognize the infection status of pseudorabies wild virus in scaled swine farms in Zhengzhou City of Henan Province in 2018,gE antibodies were tested by ELISIA towards 36 different scaled farms under fixed-point monitoring that were immunized with gE-deleted vaccines. During the period from January to December of 2018,a total of 1 057 serum samples were detected,involving 27 positive farms with the positive rate of 75.0%(27/36);309 positive samples were detected with the positive rate of 29.2%(309/1 057). Results showed that the spread of pseudorabies wild virus in Zhengzhou City had a large range,the prevalence rate was high,and the outbreak risk was high. The positive rate of gE antibody(69.8%)in the farms with 500 pigs or less was 3.65 times higher than that of the scaled farms(19.1%)with more than 3 000 pigs,which indicated that the smaller the farm was,the greater the risk of pseudorabies wild virus infection became. The gE-positive samples were tested by fluorescent PCR,and 32 positive samples were detected with the detection rate of 35.6%(32/90),which indicated that the phenomenon of virus carried or expelled by infected pigs was serious. Therefore,it was recommended that immunization with gE-deleted vaccines and gE antibody detection should be strengthened in large-scale swine farms to weed out the positive pigs intime,meanwhile,porcine pseudorabies would be gradually controlled and purified by comprehensive technologies,including isolation and disinfection.

关 键 词：伪狂犬病 猪 野毒感染 抗体检测 

分 类 号：S851.3[农业科学—预防兽医学]