淫羊藿苷对大鼠皮瓣缺血再灌注损伤后炎症反应的抑制作用  

Inhibits effect of icariin on the inflammatory response after ischemia-reperfusion injury of rat skin flap

作　　者：刘昌雄[1] 黄雄杰[1] 肖湘君[1] 王九松 LIU Chang-xiong;HUANG Xiong-jie;XIAO Xiang-jun;WANG Jiu-song(Department of Hand Foot Surgery,Affiliated Nanhua Hospital, University ofSouth China, Hengyang 421002, HunanProvince, China)

机构地区：[1]南华大学附属南华医院手足外科,湖南衡阳421002

出　　处：《中国临床药理学杂志》2019年第6期532-535,共4页The Chinese Journal of Clinical Pharmacology

基　　金：湖南省卫计委科研计划重点基金资助项目(A2017018)

摘　　要：目的研究淫羊藿苷对大鼠皮瓣缺血再灌注损伤(IRI)后的炎症反应以及对核因子κB(NF-κB)信号通路的影响。方法按照体重将大鼠随机分为5组:假手术组、模型组和低、中、高3个剂量实验组,每组8只。除假手术组,其余各组均制作大鼠腹部皮瓣IRI模型。术后,假手术组和模型组大鼠腹腔注射0. 9%Na Cl 5. 0 m L·kg^(-1)·d^(-1);低、中、高3个剂量实验组大鼠分别腹腔注射淫羊藿苷20,40,80 mg·kg^(-1)·d^(-1),连续7 d。术后7 d,测定大鼠皮瓣存活率;以酶联免疫吸附测定法检测血清中肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)和白细胞介素-6(IL-6)含量;以免疫印迹法检测皮瓣组织中磷酸化NF-κB(p-NF-κB)蛋白的表达水平。结果术后7 d,假手术组、模型组和低、中、高3个剂量实验组大鼠皮瓣的存活率分别为(91. 72±9. 64)%,(53. 81±14. 38)%,(64. 93±10. 23)%,(73. 49±11. 14)%和(85. 29±13. 72)%,这5组的血清TNF-α含量分别为(67. 35±11. 25),(153. 65±28. 11),(121. 07±22. 21),(107. 75±15. 37)和(82. 36±17. 25)ng·L-1;这5组的血清IL-1β含量分别为(34. 23±10. 24),(97. 81±18. 36),(82. 55±16. 17),(67. 91±12. 77)和(40. 35±6. 47) ng·L-1,这5组的血清IL-6含量分别为(121. 19±30. 24),(357. 32±47. 14),(282. 58±23. 68),(220. 24±31. 22)和(170. 94±21. 91) ng·L-1,这5组皮瓣组织中p-NF-κB蛋白相对表达量分别为0. 10±0. 02,0. 41±0. 09,0. 29±0. 04,0. 21±0. 03和0. 13±0. 02。模型组与假手术组比较,或者低、中、高3个剂量实验组与模型组比较,上述指标差异均有统计学意义(P <0. 05,P <0. 01)。结论淫羊藿苷可以通过抑制NF-κB信号通路激活,减少炎症因子TNF-α、IL-1β和IL-6水平,发挥减轻皮瓣IRI的作用。Objective To investigate the effects of icariin on inflammatory response and nuclear factor kappa-B( NF-κB) signaling pathway after ischemia-reperfusion injury( IRI) of rat skin flap.Methods According to the weight,rats were divided into 5 groups: sham group,model group and low,medium and high dose experimental groups,8 rats in each group.Except sham group,the rats in other groups were used to construct the abdominal flap IRI model.After operation,rats in the sham group and the model group were intraperitoneally injected with 0.9%normal saline 5.0 mL·kg^-1·d^-1,and the rats in three dose experimental groups were intraperitoneally injected with icariin 20,40,80 mg·kg^-1·d^-1 for 7 d.The survival rate of the flaps was measured 7 d after operation.The content of tumor necrosis factor-α( TNF-α),interleukin-1β( IL-1β) and interleukin-6( IL-6) in serum were detected by enzyme-linked immunosorbent assay.The protein expression level of phosphorylated NF-κB( p-NF-κB) was detected by Western blot.Results Seven days after operation,the survival rates of the flaps in the sham group,model group and low,medium and high dose experimental groups were( 91.72±9.64)%,( 53.81±14.38)%,( 64.93±10.23)%,( 73.49±11.14)%,( 85.29±13.72)%,respectively;the expression levels of serum TNF-α in these 5 groups were( 67.35±11.25),( 153.65±28.11),( 121.07±22.21),( 107.75±15.37),( 82.36±17.25) ng·L^-1,respectively;the expression levels of serum IL-1β in these 5 groups were( 34.23±10.24),( 97.81±18.36),( 82.55±16.17),( 67.91±12.77),( 40.35±6.47) ng·L^-1,respectively;the expression levels of serum IL-6 in these 5 groups were( 121.19±30.24),( 357.32±47.14),( 282.58±23.68),( 220.24±31.22),( 170.94±21.91) ng·L^-1,respectively;the relative protein expression level of p-NF-κB protein in the 5 groups of flaps tissue were 0.10±0.02,0.41±0.09,0.29±0.04,0.21±0.03,0.13±0.02,respectively.Comparison between model group and sham operation group,or comparison between three dose experimental groups and model group,differenc

关 键 词：淫羊藿苷 皮瓣 缺血再灌注损伤 炎症 核因子kB信号通路 

分 类 号：R28[医药卫生—中药学]