藏羊巨型住肉孢子虫体外培养方法的建立  

Establishment of Culture Method in Vitro of Sarcocystis gigantea from Tibetan Sheep

作　　者：苏晓雪 康明[1] SU Xiao-xue;KANG Ming(College of Agriculture and Animal Husbandry,Qinghai University,Xining,Qinghai 810016,China)

机构地区：[1]青海大学农牧学院,青海西宁810016

出　　处：《动物医学进展》2019年第4期32-35,共4页Progress In Veterinary Medicine

基　　金：国家自然科学基金项目(31660698);青海省农牧厅项目(NMSY-2016-01)

摘　　要：为了建立青海藏羊巨型住肉孢子虫体外培养方法,以贴壁生长和形态完整的非洲绿猴肾细胞(Vero)作为宿主细胞,将青海藏羊巨型住肉孢子虫缓殖子(1.25×104个/mL)接种于宿主细胞,连续培养21d,期间观察记录其生长发育状况。结果表明,藏羊巨型住肉孢子虫在Vero细胞中生长良好,并在培养液中观察到游离的虫体卵囊,21d后均可分离、纯化出新一代的缓殖子。说明在实验室条件下,成功建立了藏羊巨型住肉孢子虫稳定的体外培养方法。This paper studied culture methord in vitro for Sarcocystis giganteafrom Qinghai Tibetan sheep using adherent and intact African green monkey kidney cells(Vero)as host cells.Qinghai Tibetan sheep Sarcocystis gigantea bradyzoites(1.25×10^4/mL)were inoculated into the host cells and cultured for twenty-one consecutive days.The growth and development of the host cells were observed and recorded by using a microscopic imaging system.The experimental results showed that the Sarcocystis gigantea of Tibetan sheep grew well in Vero cells,and free oocysts were observed in the culture solution,the new generation of bradyzoites was able to be isolated and purified after twenty-one days.A stable culture in vitro method for Sarcocystis giganteafrom Tibetan sheep was successfully established under laboratory conditions.

关 键 词：青海藏羊 非洲绿猴肾细胞 巨型住肉孢子虫 体外培养 

分 类 号：S852.723[农业科学—基础兽医学] S858.26[农业科学—兽医学]