1,25-二羟基维生素D_3对脂肪细胞脂滴代谢的影响  

Effect of 1,25-dihydroxy vitamin D3 on lipid droplet metabolism in lipocyte

作　　者：向薇 程实 马玲 XIANG Wei;CHENG Shi;MA Ling(College of Public Health,Xinjiang Medical University,Urumqi,Xinjiang 830011,China;Teaching and Researching Section of Nutrition and Food Health,College of Public Health,Southwest Medical University,Luzhou,Sichuan 646000,China)

机构地区：[1]新疆医科大学公共卫生学院,乌鲁木齐830011 [2]西南医科大学公共卫生学院营养与食品卫生学教研室,四川泸州646000

出　　处：《重庆医学》2019年第6期937-941,共5页Chongqing medicine

摘　　要：目的探索1,25-二羟基维生素D_3[1,25(OH)_2D_3]对棕榈酸(PA)诱导的分化后的3T3-L1细胞脂滴代谢的影响。方法用"鸡尾酒法"对正常培养的3T3-L1细胞进行成脂诱导分化,8d后分别用100、300、600、900μmol/L PA进行24h造模干预,MTT和三酰甘油(TG)水平的检测筛选最佳造模浓度。在模型基础上分别用1、10、100nmol/L的1,25(OH)_2D_3进行干预,24h后检测各组细胞TG水平,对细胞进行油红O染色,计算脂滴数量和平均直径,检测细胞脂滴代谢相关基因(PPAR-α、PPAR-γ、CIDE-a、CIDE-c、UCP-1和PLIN-1)mRNA表达。结果与模型组比较,10、100nmol/L的1,25(OH)_2D_3明显降低脂滴平均直径,增加脂滴数量,上调了PPAR-α和PLIN-1 mRNA表达,下调CIDE-a、CIDE-c mRNA表达。1nmol/L的1,25(OH)_2D_3在24h的干预内并没有明显改变脂滴数量和平均直径,以及相关基因表达。结论 10、100nmol/L浓度的1,25(OH)_2D_3可以缓解PA造成分化后的3T3-L1细胞肥大。Objective To explore the effect of 1,25-dihydroxy vitamin D3[1,25(OH)2D3]on the lipid droplet metabolism in differentiated 3T3-L1cells induced by palmitic acid(PA).Methods The cocktail method was used to conduct the adipogensis differentiation in normally cultured 3T3-L1cells.The 24hmodeling intervention was conducted after 8dby 100,300,600,900μmol/L PA.The control group was set.The best modeling concentration was screened by MTT and TG level detection.Under the model basis,the intervention was performed by 1,10,100nmol/L 1,25(OH)2D3.The blank group and model group were set.Then The TG level in the cells of each group was detected after 24h.The cells were stained by oil red,and the mean diameter and quantity of lipid droplets(LD)were calculated by using the Image-pro plus 6.0.Then the mRNA expression of cell lipid droplet metabolic related genes(PPAR-α,PPAR-γ,CIDE-a,CIDE-c,UCP-1and PLIN-1)were detected.Results Compared with model group,10,100nmol/L concentrations of 1,25(OH)2D3 decreased the average diameter of intracellular lipid droplet,increased the quantity of lipid droplet and related gene expression,up-regulated the mRNA expression levels of PPAR-αand PLIN-1,and down-regulated the mRNA expression levels of CIDE-a and CIDE-c.However,1nmol/L 1,25(OH)2D3did not obviously alter the content and morphology of lipid droplet as well as the expression of related genes.Conclusion 10,100nmol/L1,25(OH)2D3can alleviate the hypertrophy of differentiated 3T3-L1cells caused by PA.

关 键 词：肥大细胞 骨化三醇 脂滴 

分 类 号：R34[医药卫生—基础医学]