大鼠不同胚层来源成骨细胞生物学行为比较研究  

Comparison of the biological behaviors of rat osteoblasts from distinct embryonic germ layers

作　　者：陈玉玲[1] 许雄程 江俊[1] 阳雪 吴玉铭 何梦娇[1] 骆凯[1] CHEN Yuling;XU Xiongcheng;JIANG Jun;YANG Xue;WU Yuming;HE Mengjiao;LUO Kai(School and Hospital of Stomatology,Institute of Stomatology,Fujian Medical University & Key Liboratory of Stomatology,Fujian Province University,350002 Fuzhou,China)

机构地区：[1]福建医科大学附属口腔医院福建医科大学口腔医学研究院福建省高校口腔医学重点实验室,福州350002

出　　处：《实用口腔医学杂志》2019年第2期196-200,共5页Journal of Practical Stomatology

基　　金：国家自然科学基金(编号:81100761);福建省科技创新联合资金项目(编号:2016Y9023);福建省卫生系统中青年骨干人才培养项目(编号:2015-ZQN-ZD-28)

摘　　要：目的:探讨大鼠不同胚层来源成骨细胞生物学行为的差异。方法:体外培养大鼠颌骨与股骨成骨细胞(MOBs与FOBs),通过MTT比色法检测细胞的增殖能力,采用碱性磷酸酶染色与活性测定、茜素红染色与定量比较细胞的成骨能力。应用荧光定量PCR及ELISA检测细胞成骨与成血管相关细胞因子的表达情况。结果:与FOBs相比, MOBs的增殖能力、成血管相关细胞因子Angpt-1和FGF-2的表达更强,但碱性磷酸酶的表达、体外矿化结节形成能力以及成骨相关基因ALP、RUNX2、Col-Iα1和OC的表达均较低。结论:MOBs与FOBs相比较,增殖力较强,分化能力较弱。Objective:To study the difference of the biological behaviors of rat osteoblasts from distinct embryonic germ layers.Methods:Osteoblasts were isolated and cultured from mandible(MOBs)and femur(FOBs)of rats respectively.The proliferation of the cells was assessed by MTT assay.The property of osteogenic differentiation was evaluated by ALP staining and,Alizarin red staining and quantification analysis.The expression of osteogenesis-related genes and angiogenesis-related cytokines of the cells was evaluated by Real-time PCR and ELISA respectively.Results:Compared FOBs,MOBs showed higher proliferation ability,up-regulated the expression of angiogenesis-related cytokines Angpt-1 and FGF-2,but lower ALP activity and lower formation of mineralized nodules and lower expression of osteogenesis-related genes ALP,RUNX2,Col-Iαand OC.Conclusion:MOBs may have stronger and lower proliferation ability,lower osteogenic differentiation and pro-angiogenic property than FOBs.

关 键 词：颌骨 股骨 成骨细胞 成骨 成血管 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]