脊髓背角P物质、ERK1/CREB信号通路激活在大鼠椎间盘源性内脏痛形成机制中的作用  

Effect of substance P and ERK1/CREB signaling pathway activation in spinal dorsal horn on the mechanism of discogenic visceral pain development in rats

作　　者：唐元章[1] 孙晨力 郭玉娜[1] 杨立强[1] 武百山[1] 魏亚 倪家骧[1] TANG Yuanzhang;SUN Chenli;GUO Yu′na;YANG Liqiang;WU Baishan;WEI Ya;NI Jiaxiang(Department of Pain Management, Xuanwu Hospital, Capital Medical University, Beijing 100053, China)

机构地区：[1]首都医科大学宣武医院疼痛科,北京100053

出　　处：《中国医药导报》2019年第10期7-10,共4页China Medical Herald

基　　金：北京市医院管理局青年人才培养"青苗"计划项目(QML20160807);首都医科大学基础-临床科研合作基金课题(16JL43)

摘　　要：目的探讨大鼠自体退变髓核注射导致椎间盘源性内脏痛的发生机制。方法将24只雄性SD大鼠按照随机数字表法分为空白对照组、假手术组、髓核注射组,每组8只。髓核注射组大鼠在X线下行右侧腰交感干自体退变髓核悬液注射,假手术组注射同等剂量生理盐水,空白对照组不做任何处理。注射后14 d收集各组大鼠L_(1～3)脊髓节段,采用免疫印迹法半定量分析P物质(SP)和细胞外调节蛋白激酶1/2 (ERK1/2)、p-ERK1/2和c AMP反应元件蛋白(CREB)、p-CREB表达的变化。结果髓核注射组大鼠脊髓SP及p-ERK1、p-CREB表达较假手术组及空白对照组明显增多(P <0.05),三组p-ERK2表达比较,差异无统计学意义(P> 0.05)。结论大鼠自体退变髓核注射导致椎间盘源性内脏痛的产生可能与相应脊髓节段SP释放及ERK1/CREB磷酸化信号传导通路的激活有关。Objective To investigate the molecular mechanisms of discogenic visceral pain resulted from autologous degenerative nucleus pulposus (NP) injection. Methods A total of 24 male SD rats were divided into naive group (n = 8), sham group (n = 8) and NP-treated group (n = 8) according to random number table method, with 8 rats in each group. Under fluoroscopic, autologous degenerative NP suspension was injected into right sympathetic trunk of rats in NP-treated group and the same dose of saline was administrated to right sympathetic trunk of animals in sham group, while the rats in the naive group did not receive any treatment. After 14 days postoperatively, the expression of substance P (SP), extracellular regulated protein kinases 1/2 (ERK1/2), p-ERK1/2, cyclic AMP response element binding protein (CREB) and p-CREB in the L1-L3 spinal cord of each group was analyzed by Western blot. Results The SP, p-ERK1, p-CREB expression in NP-treated group was significantly increased compared with those in naive group and control group (P < 0.05), while there was no significant difference in p-ERK2 expression among the three groups (P > 0.05). Conclusion The development of discogenic visceral pain induced by autologous degenerative NP injection is concerned with SP release and p-ERK1/CREB signaling pathway activation.

关 键 词：脊髓背角 内脏痛 椎间盘 大鼠 信号通路 

分 类 号：R338.21[医药卫生—人体生理学]