基于超滤-络合萃取技术的甘草酸制备工艺研究  

Preparation technology of glycyrrhizin acid based on ultrafiltrationcomplexation extraction technology

作　　者：周博 蒲红利 王继龙[1] 刘晓霞[1] 魏舒畅[1] 石盘棋 孙晓燕[1] ZHOU Bo;PU Hong-li;WANG Ji-long;LIU Xiao-xia;WEI Shu-chang;SHI Pan-qi;SUN Xiao-yan(Research Center of Traditional Chinese Medicine Pharmaceutical Technology and Engineering of Gansu Province, Gansu University of Chinese Medicine, Lanzhou 730000, China)

机构地区：[1]甘肃中医药大学甘肃省中药制药工艺工程研究中心,甘肃兰州730000

出　　处：《中草药》2019年第6期1323-1327,共5页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81460608);甘肃省基础研究创新群体项目(1506RJIA034)

摘　　要：目的建立一种采用超滤-络合萃取技术制备甘草酸的工艺。方法考察pH值(p H 4～8)对超滤液中甘草酸萃取率的影响;采用正交试验优选络合萃取甘草酸的工艺条件;通过考察反萃取剂种类及浓度,确定甘草酸的反萃取工艺条件。结果所得甘草酸最佳络合萃取工艺条件为超滤液pH值应调至2、萃取剂为三烷基氧化膦(TRPO)-磺化煤油(5∶95)、有机相与水相体积比为1∶1,甘草酸平均萃取率达到99.2%。甘草酸最佳反萃取工艺条件为22.5 mmol/L NaOH水溶液为反萃取剂、有机相与反萃取剂体积比1∶1,甘草酸单次反萃取率达到98.8%,甘草酸总转移率为98.1%。结论超滤-络合萃取技术可作为甘草酸制备的一种新工艺。Objective To establish a technique for preparing glycyrrhizin acid(GA) by ultrafiltration-complexation extraction.Methods The effect of pH on the extraction rate of GA from ultrafiltrate was investigated in the range of 4-8;The orthogonal test was used to optimize the technological conditions for complexation extraction of GA;The extraction conditions of GA were determined by investigating the type and concentration of the extractant. Results The optimum extraction conditions for GA were as follows: pH value of Glycyrrhiza uralensis ultrafiltrate was adjusted to 2, TRPO-sulfonated kerosene(5∶95, volume percent), volume ratio of organic phase to aqueous phase was 1∶1, and average extraction rate of GA reached 99.2%. The best back extraction conditions for GA were as follows: 22.5 mmol/L NaOH aqueous solution was used as the stripping agent, the volume ratio of organic phase to stripping agent was 1∶1, the single back extraction rate of GA reached 98.8%, and the total transfer rate of GA was 98.1%.Conclusion Ultrafiltration-complexation extraction technology can be used as a new process for the preparation of GA.

关 键 词：络合萃取 反萃取 甘草酸 三烷基氧化膦(TRPO) 甘草超滤液 

分 类 号：R284.2[医药卫生—中药学]