绵马贯众炭中新型碳点的发现及其止血作用研究  

Novel carbon dots derived from Dryopteridis Crassirhizomatis Rhizoma Carbonisatum and their hemostatic effect

作　　者：熊威 赵琰 成金俊 张美龄 孙紫薇 罗娟 朱雅凡 张亚雪 孔慧 屈会化[3] XIONG Wei;ZHAO Yan;CHENG Jin-jun;ZHANG Mei-ling;SUN Zi-wei;LUO Juan;ZHU Ya-fan;ZHANG Ya-xue;KONG Hui;QU Hui-hua(School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China;College of Traditional Chinese Medicine, Beijing University of Traditional Chinese Medicine, Beijing 100029, China;Beijing Institute of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China)

机构地区：[1]北京中医药大学中药学院,北京100029 [2]北京中医药大学中医学院,北京100029 [3]北京中医药大学中医药研究院,北京100029

出　　处：《中草药》2019年第6期1388-1394,共7页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81573573);国家自然科学基金资助项目(81503344);国家自然科学基金资助项目(81473338)

摘　　要：目的在绵马贯众炭中发现新型的纳米类成分——碳点,并研究其止血作用。方法通过对绵马贯众炭水煎液提取、分离和透析得到一种新型水溶性的纳米类成分碳点,命名为绵马贯众炭碳点(DCC-CDs)。利用透射电子显微镜(TEM)、高分辨透射电子显微镜(HR-TEM)、紫外-可见分光光度法(UV-vis)、傅里叶变换红外光谱(FT-IR)、X-射线光电子能谱分析(XPS)、高效液相色谱(HPLC)等多种仪器和方法,对DCC-CDs进行了表征。通过小鼠断尾和肝脏出血实验评价DCC-CDs的止血作用,通过测定凝血四项及血小板(PLT)数量探讨其止血机制。结果从绵马贯众炭中分离出来的DCC-CDs外形近似球形,分散度良好,粒径分布在1～7 nm。动物实验结果显示DCC-CDs具有良好的止血效果,能显著减少小鼠断尾和肝脏出血时间(P<0.01)。此外,DDC-CDs也能使大鼠血液中纤维蛋白原(FIB)浓度和PLT的数量显著升高(P<0.05)。结论 DCC-CDs具有止血效果,本研究为应用DCC-CDs治疗出血性疾病的研究提供新思路,也为中药有效成分的探索提供全新的思维模式。Objective A new type of nanoparticles-carbon dots was identified from Dryopteridis Crassirhizomatis Rhizoma Carbonisatum(DCC) and investigated their hemostatic effect. Methods DCC was boiled, filtered, and dialyzed to separate and obtain a novel type of carbon dots named DCC-CDs. These DCC-CDs were characterized using transmission electron microscopy(TEM) and high-resolution TEM, as well as Fourier transform infrared, ultraviolet-visible, X-ray photoelectron spectroscopy, and HPLC. Then, the anti-hemorrhagic effects were evaluated by severed tails ad liver bleeding tests and the related hemostatic mechanisms of the obtained DCC-CDs were assessed by detecting blood coagulation and PLT quantity to discuss the hemostasis mechanism. Results The DCC-CDs separated from DCC were spherical, monodisperse, which had a narrow size distribution between 1 and 7 nm. In the animal experiment, DCC-CDs were revealed excellent hemostatic bioactivity, and significant decreased in the tail and liver bleeding time(P <0.01). Additionally, the rats showed a profound increasing in fibrinogen(FIB) and platelets(PLT) after DCC-CDs treatment(P < 0.05).Conclusion These results demonstrated an explicit hemostatic effect of DCC-CDs, which offers new avenues for research into DCC to treat hemorrhagic diseases and a new mindset for the exploration of the active ingredients of traditional Chinese medicine.

关 键 词：绵马贯众炭 碳点 表征 止血作用 止血机制 

分 类 号：R285.5[医药卫生—中药学]