砂贝母中异甾体生物碱类成分的研究  

Isosteroidal Alkaloids from Fritillaria karelinii

作　　者：黄金昌 雷春 阿吉艾克拜尔·艾萨[2] 俞媚华 阿布力米提·伊力[2] 侯爱君 Huang Jinchang;Lei Chun;Aisa Haji Akber;Yu Meihua;Yili Abulimiti;Hou Aijun(Department of Pharmacognosy,School of Pharmacy,Fudan University,Shanghai 201203;Key Laboratory of Plant Resources and Chemistry in Arid Zone and State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization,Xinjiang Technical Institute of Physics and Chemistry,Chinese Academy of Sciences,Urumqi 830011)

机构地区：[1]复旦大学药学院生药学教研室,上海201203 [2]中国科学院新疆理化技术研究所中国科学院干旱区植物资源化学重点实验室新疆特有药用资源利用重点实验室,乌鲁木齐830011

出　　处：《有机化学》2019年第3期842-847,共6页Chinese Journal of Organic Chemistry

基　　金：国家自然科学基金新疆联合基金(No.U1403201)资助项目~~

摘　　要：利用各种柱色谱和高效液相色谱等分离纯化方法,从砂贝母鳞茎中分离得到 6 个异甾体生物碱类化合物.根据质谱、一维/二维核磁共振谱和 X 射线单晶衍射等技术鉴定了它们的结构,分别为 karelinine (1), 5-epikarelinine (2),27-epiebeienine (3), ebeienine (4), persicanidine B (5)和 heilonine (6),其中化合物 1~3 为新化合物.化合物 1 是贝母属中罕见的 5β-jervine 型异甾体生物碱,具有 A/B 环顺式稠合方式.化合物 1 和 2 也是贝母属中首次发现的具有 15α-OH 取代的 jervine 型生物碱.Three new isosteroidal alkaloids, karelinine (1), 5-epikarelinine (2), and 27-epiebeienine (3), were isolated from the bulbs of Fritillaria karelinii by column chromatography and semipreparative HPLC (high performance liquid chromatography), together with three known ones, ebeienine (4), persicanidine B (5), and heilonine (6). Their structures were identified by MS, 1D/2D NMR, and single-crystal X-ray diffraction. Compound 1 is a 5β-jervine isosteroidal alkaloid featuring a cis-fused A/B ring moiety, rarely found in the Fritillaria genus. Compounds 1 and 2 also represent the first jervine alkaloids with a 15 α-hydroxy group from this genus.

关 键 词：砂贝母 异甾体生物碱 karelinine 5-epikarelinine 27-epiebeienine 

分 类 号：R284[医药卫生—中药学] O657.7[医药卫生—中医学]