高效薄层色谱扫描法同时测定滁菊中木犀草素、芹菜素、金合欢素含量  

Simultaneous Determination of Luteolin,Apigenin and Acacetin in Chujuby HTLCS Methods

作　　者：蔡玉茹 江倩 李洒洒 冯顺丽 周国梁 CAI Yuru;JIANG Qian;LI Sasa;FENG Shunli;ZHOU Guoliang(Collage of Life and Health Science,Anhui Science and Technology University,Fengyang 233100,China)

机构地区：[1]安徽科技学院生命与健康科学学院,安徽凤阳233100

出　　处：《安徽科技学院学报》2019年第1期51-55,共5页Journal of Anhui Science and Technology University

基　　金：安徽省高校省级自然科学研究重点项目(KJ2014A051);安徽省教育厅重点和重大项目(KJ2016SD16);安徽省大学生创新创业训练计划项目(2017S10879004;2017S10879006)

摘　　要：目的:采用高效薄层色谱扫描法(HTLCS)同时测定滁菊中木犀草素、芹菜素和金合欢素的含量。方法:采用硅胶GF254高效薄层板条带点样,以甲苯∶乙酸乙酯∶甲酸∶水=17∶7∶3∶2上层溶液为展开剂,上行展开,于365nm波长下进行直线扫描,测定滁菊中的木犀草素、芹菜素、金合欢素的含量。结果:分别在0.066 1～0.335 2μg(r=0.999 9),0.068 4～0.333 8μg(r=0.997 9)、0.064 0～0.334 2μg(r=0.995 2)范围内,木犀草素、芹菜素、金合欢素线性关系良好;平均回收率分别为99.9%、100.65%、101.3%,RSD分别为1.55%、1.41%、1.64%。结论:以HTLCS为质量控制方法,建立了一种简便、快速、准确的药物检测方法。Objective:To establish a method for determining the concentrantion of luteolin,apigenin and acacetin in Chuju by HTLCS.Methods:To determine the concentration of luteolin,apigenin,cacetin in chuju by linear scanning at the wavelength of 365 nm,the sample was separated by using the supernatant of toluene∶ethyl acetate∶formic acid∶water=17∶7∶3∶2 on the silica GF 254 plates.Results:The linear range were 0.066 1-0.335 2μg for luteolin(r=0.999 9),0.068 4-0.333 8μg for apigenin(r=0.997 9)and 0.064 0-0.334 2μg for acacetin(r=0.995 2),respectively in HTLCS.The average recoveries of luteolin,apigenin and acacetin were 99.9%,100.65%,101.3%.The RSD were 1.55%,1.41%,1.64%,respectivly.Conclusion:A simple and accurate platform for the Chuju drug assay using HTLCS method as quality control.

关 键 词：滁菊 木犀草素 芹菜素 金合欢素 薄层扫描法 

分 类 号：R917[医药卫生—药物分析学]