肝癌小鼠血清荧光谱峰位蓝移及分子振动机理研究  

The study of the peak position blue shift and molecular vibration mechanism of serum fluorescence spectra in mice with liver cancer

作　　者：关岑岑 骆晓森[1] 陆健[1] 李泽文 GUAN Cen-cen;LUO Xiao-sen;LU Jian;Li Ze-wen(Faculty of Science,Nanjing University of Science and Technology ,Nanjing 210094,China)

机构地区：[1]南京理工大学理学院,江苏南京210094

出　　处：《光电子．激光》2019年第2期221-226,共6页Journal of Optoelectronics·Laser

基　　金：国家自然科学基金(61605079);江苏省自然科学基金(BK20130751)资助项目

摘　　要：通过多峰高斯拟合方法,对接种了肝癌小鼠生长5～15天期间的血清荧光光谱进行系统研究,结果表明,随着肿瘤的生长,荧光谱曲线及其高斯基元函数的峰值位置都有向短波方向移动的趋势。基于分子振动理论的分析认为,是肿瘤生长过程中,血清中部分发光分子的含量增加,其分子趋于彼此靠近,分子间的相互作用增强,分子中原子间化学键趋于伸长,其基态的振动能级趋于降低,若第一电子激发态的最低振动能级未发生变化,则基态与第一电子激发态最低振动能级之间的能量差趋于增大,导致相关荧光谱曲线的峰值位置趋于蓝移。本文的研究结果表明,对血清荧光光谱进行非线性多峰拟合及分子振动理论分析,有助于从分子层面揭示血清中荧光物质变化的深层次原因。By using the method of multi-peak Gaussian fitting,the fluorescence spectra of serum from 5-15 days of liver cancer mice were studied,and the results show that with the growth of tumor,the peak position of the fluorescence composing line and its Gaussian function move to short wave direction. Based on the theory of molecular vibration,it is believed that in the course of tumor growth,the content of some luminous molecules in the serum increases,the molecules tend to be close to each other,the interaction between the molecules is enhanced,the chemical bond between the atoms tends to elongate,and the vibrational energy level of the atomic state is reduced. If the lowest vibrational level of the first electron excitation state is not changed,the energy difference between the ground state and the lowest vibrational level of the first electron excited states tends to increase,resulting in the peak position of the correlated fluorescence spectral curve becoming blue shift. The results of this study show that the nonlinear multi-peak fitting and molecular vibration analysis of serum fluorescence spectra are helpful to reveal the deep-seated causes of the changes of fluorescent substances in serum from the molecular level.

关 键 词：肝癌 血清 荧光光谱 峰位蓝移 高斯拟合 能级跃迁 

分 类 号：Q631[生物学—生物物理学]