两种脉络膜血管铺片法对实验性脉络膜新生血管定量研究的比较  

Comparison of quantitative study for experimental choroidal neovascularization using two methods of choroid flat mounts

作　　者：焦剑[1] 莫宾 刘璐[3] 华文[1] JIAO Jian;MO Bin;LIU Lu;HUA Wen(Department of Ophthalmology,Beijing Chaoyang Hospital,Capital Medical University,Beijing Key Laboratory of Ophthalmology and Visual Science,Beijing 100020,China;Beijing Tongren Eye Center,Beijing Tongren Hospital,Capital Medical University,Beijing Key Laboratory of Ophthalmology and Visual Science,Beijing 100730,China;Department of Ophthalmology,Fuxing Hospital,Capital Medical University,Beijing Key Laboratory of Ophthalmology and Visual Science,Beijing 100038,China)

机构地区：[1]首都医科大学附属北京朝阳医院眼科眼科学与视觉科学北京市重点实验室,100020 [2]首都医科大学附属北京同仁医院北京同仁眼科中心眼科学与视觉科学北京市重点实验室,100730 [3]首都医科大学附属北京复兴医院眼科眼科学与视觉科学北京市重点实验室,100038

出　　处：《眼科》2019年第2期120-124,共5页Ophthalmology in China

基　　金：首都医科大学眼科学与视觉科学北京市重点实验室开放研究课题资助项目(2015YKSJ03)

摘　　要：目的比较两种脉络膜血管铺片法对实验性脉络膜新生血管(CNV)进行定量研究的效果。设计实验研究。研究对象BN大鼠。方法正常BN大鼠24只随机分为两组,采用相同直径激光斑诱导CNV模型,于光凝后1、2、4周分别采用荧光素异硫氰酸酯-葡聚糖(FITC-D)灌注法及凝集素法进行脉络膜血管铺片,激光共聚焦显微镜照相,Image J图像分析软件测量CNV面积。以BN大鼠麻醉开始,到脉络膜血管铺片结束计时,计算两种方法所需实验时间。以BN大鼠进行脉络膜血管铺片所消耗的FITC-D或者凝集素的剂量,计算每只标本实验费用。主要指标CNV面积、实验时间及实验费用。结果 BN大鼠光凝后1、2、4周,FITC-D灌注法及凝集素法的CNV面积(μm^2)分别为5524±1847、50653±7265、121623±7128及14560±4508、144209±8411、204066±8546;实验时间(单位:分钟)分别为119±21及390±34;实验费用(单位:元)分别289±26.3及32±4.2。结论两种方法因实验原理不同,造成CNV面积、实验时间及实验费用等方面均存在差异,实验者可以根据各自实验目的选择相应方法进行CNV相关研究。(眼科,2019, 28:120-124)Objective To compare two methods of choroid flat mounts in quantitative study for experimental choroidal neovascularization (CNV). Design Experimental study. Participants Brown-Norway (BN) rats. Methods Normal 24 BN rats were randomly divided into two groups equally and CNV models were induced by laser photocoagulation with the same diameter spot. Choroid flat mounts were performed with fluorescein isothiocyanate-dextran (FITC-D) perfusion method or isolectin method at 1 week(W), 2W, and 4W after photocoagulation respectively. The photo of choroid flat mounts was taken under laser confocal microscope and the area of CNV was measured with Image J software. The experimental time was recorded form the start of anaesthesia to the end of choroid flat mounts. The expense of one choroid flat mount was calculated by the dose of FITC-D or lectin consumed in the experiment. Main Outcome Measures Area of CNV, experimental time and expense. Results At 1W, 2W, and 4W after photocoagulation, the areas (μm^2) of CNV by FITC-D perfusion method or isolectin method were 5524±1847, 50653±7265, 121623±7128 or 14560±4508, 144209±8411, 204066±8546 respectively. The experimental time by two different methods was 119±21min or 390±34min respectively, while the experimental expense was 289±26.3￥ or 32±4.2￥ respectively. Conclusion There were obvious differences in the area of CNV, experimental time and expense between the 2 methods of choroid flat mounts. The appropriate method of choroid flat mounts for experimental CNV should be carefully selected according to the research purposes.

关 键 词：脉络膜新生血管 动物模型 脉络膜血管铺片 面积 

分 类 号：R773.4[医药卫生—眼科]