栀子苷对酵母多糖致大鼠肠黏膜屏障损害作用的研究  

Effects of Geniposide on Intestinal Mucosal Barrier Damage Induced by Zymosan in Rats

作　　者：邓怒骄 陈凌波[1] 谭瑛子 兰斌 邓常清[1] 张文兴[1] DENG Nujiao;CHEN Lingbo;TAN Yingzi;LAN Bin;DENG Changqing;ZHANG Wenxing(Hunan University of Chinese Medicine, Changsha, Hunan 410208, China)

机构地区：[1]湖南中医药大学,湖南长沙410208

出　　处：《湖南中医药大学学报》2019年第4期465-469,共5页Journal of Hunan University of Chinese Medicine

基　　金：国家自然科学基金资助项目(81473581)

摘　　要：目的探讨栀子苷对酵母多糖(zymosan, Zym)致大鼠肠黏膜屏障损害的作用。方法通过腹腔注射Zym-石蜡混悬液建立大鼠肠黏膜屏障损害模型,将模型大鼠随机分为模型组、栀子苷组、乌司他丁组,腹腔注射Zym后即刻干预,12 h后重复干预1次。另设正常对照组。造模24 h后测定大鼠血清肿瘤坏死因子-α(tumor necrosis factor-α, TNF-α)、白介素-1β(interleukin-1β, IL-1β)、白介素-6(interleukin-6, IL-6)、白介素-10(interleukin-10, IL-10)、D-乳酸(D-lactate, D-LA)、二胺氧化酶(diamine oxidase,DAO)、内毒素(endotoxin, ET)水平,并取小肠进行病理组织学检查。结果模型组大鼠血清TNF-α、IL-1β、IL-6、IL-10、D-LA、DAO、ET水平较正常对照组显著增高(P<0.01);病理组织学检查可见黏膜上皮水肿,黏膜上皮细胞排列紊乱,黏膜上皮炎症细胞增多,固有层松散、充血;与模型组比较,栀子苷可降低肠黏膜屏障损害大鼠血清TNF-α、IL-1β、IL-6、D-LA、DAO及ET水平,增加IL-10水平(P<0.05, P<0.01),减轻肠组织病理性损伤。结论栀子苷可以减轻Zym导致的大鼠炎症反应,对Zym引起的肠黏膜屏障损害具有保护作用。Objective To investigate the effects of geniposide on intestinal mucosal barrier damage induced by zymosan (Zym) in rats. Methods Intestinal mucosal barrier damage model of rats was induced by intraperitoneal injection of zymosan-paraffin suspension. The model rats were randomly divided into the model group, geniposide group and ulinastatin group. Drugs were given after intraperitoneal injection of zymosan-paraffin suspension immediately and 12 h later. The normal control group was set up. Then, 24 h after modeling, the levels of tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β), interleukin-6 (IL-6), interleukin-10 (IL-10), D-lactate (D-LA), diamine oxidase (DAO), and endotoxin (ET) in the serum were measured, and the intestines of rats were taken for histopathological examination. Results The levels of TNF-α, IL-1β, IL-6, IL-10, D-LA, DAO, and ET in the serum in the model group were significantly higher than those in the normal control group (P<0.01). The pathological examination showed intestinal mucosal epithelial edema, disordered arrangement of mucosal epithelial cells, increased mucosal epithelial inflammatory cells, congestive lamina propria. Geniposide could decrease the levels of TNF-α, IL-1β, IL-6, D-LA, DAO and ET, and increase the IL-10 level (P<0.05, P<0.01), reduce pathological damage in intestinal tissue. Conclusion Geniposide could reduce inflammatory reaction, attenuate the intestine mucosal damage induced by zymosan in rats.

关 键 词：栀子苷 酵母多糖 肠黏膜屏障 炎症反应 

分 类 号：S285.5[农业科学—农业水土工程]