柬埔寨柯拉斯那沉香的化学成分研究  

Chemical constituents of agarwood originating from Aquilaria crassna in Cambodia

作　　者：夏录录 李薇[2] 梅文莉[2] 杨理 蔡彩虹[2] 姜北 戴好富[2] XIA Lulu;LI Wei;MEI Wenli;YANG Li;CAI Caihong;JIANG Bei;DAI Haofu(Pharmacy and Chemistry College , Dali University , Dali 671003, Yunnan, China;Key Laboratory of Biology and Genetic Resources of Tropical Crops , Ministry of Agriculture , Institute of Tropical Bioscience and Bio-technology , Chinese Academy of Tropical Agricultural Sciences , Haikou 571101, China)

机构地区：[1]大理大学药学与化学学院,云南大理671003 [2]中国热带农业科学院热带生物技术研究所农业部热带作物生物学与遗传资源利用重点实验室,海口571101

出　　处：《广西植物》2019年第4期540-547,共8页Guihaia

基　　金：海南省自然科学基金(创新研究团队项目)(2017CXTD020);中国热带农业科学院院级创新团队项目(17CXTD-15;17CXTD-30);现代农业产业技术体系建设专项项目(CARS-21)~~

摘　　要：该文采用ODS、硅胶、Sephadex LH-20等柱色谱技术,对柬埔寨野生柯拉斯那沉香(Aquilaria crassna)进行了研究。结果表明:从柬埔寨柯拉斯那所产沉香的乙醇提取物中进行分离共得到了10个化合物,包括一对对映异构体(9a/9b)。经波谱解析分别鉴定为6-甲氧基-2-[2-(3-羟基-4-甲氧基苯)乙基]色酮(1)、6-甲氧基-2-[2-(3-甲氧基-4-羟基苯)乙基]色酮(2)、6,7-二甲氧基-2-(2-苯乙基)色酮(3)、6-羟基-2-(2-苯乙基)色酮(4)、6-羟基-2-[2-(4-甲氧基苯)乙基]色酮(5)、8-氯-6-羟基-2-[2-(3-羟基-4-甲氧基苯)乙基]色酮(6)、8-氯-6-羟基-2-[2-(4-甲氧基苯)乙基]色酮(7)、oxidoagarochromone B(8)、4'-demethoxyaqusisnenone D(9)。其中,化合物6、7和9均为首次从柯拉斯那沉香中分离得到。活性测试结果显示,化合物1和2对乙酰胆碱脂酶具有一定的抑制活性,化合物2对人慢性髓原白血病细胞K562具有较弱的抑制作用。Ten compounds, including one pair of enantiomers (9a/9b), were isolated from the ethanol extract of agarwood originating from Aquilaria crassna in Cambodia by comprehensive chromatographic techniques including ODS, silica gel and Sephadex LH-20. On the basis of spectroscopic data, they were identified as 6-methoxy-2-[2-(3-hydroxy-4-methoxyphenyl) ethyl] chromone ( 1 ), 6-methoxy-2-[2-(3-methoxy-4-hydroxyphenyl) ethyl] chromone ( 2 ), 6,7-dimethoxy-2-(2-phenylethyl) chromone ( 3 ), 6-hydroxy-2-(2-phenylethyl) chromone ( 4 ), 6-hydroxy-2-[2-(4-methoxyphenyl) ethyl ] chromone( 5 ), 8-chloro-6-hydroxy-2-[2-(3-hydroxy-4-methoxyphenyl) ethyl]chromone ( 6 ), 8-chloro-6-hydroxy-2-[2-(4-methoxyphenyl) ethyl] chromone ( 7 ), oxidoagarochromone B ( 8 ), 4′-demethoxyaqusisnenone D ( 9 ). Compounds 6 , 7 and 9 were isolated from agarwood of A. crassna for the first time. Among those, compounds 1 and 2 showed inhibitory activities against acetylcholinesterase. Compound 2 had weak inhibitory effect on K562 cell lines.

关 键 词：柬埔寨 沉香 柯拉斯那 化学成分 乙酰胆碱脂酶抑制活性 细胞毒活性 

分 类 号：Q946.91[生物学—植物学] R285.5[医药卫生—中药学]