大孔吸附树脂分离纯化棘白霉素B母核的工艺研究  

Separation and Purification of Echinocandin B Nucleus by Macroporous Adsorption Resin

作　　者：彭亮[1] 王晶珂[1] 谢新宇[1] 李敏[1] PENG Liang;WANG Jingke;XIE Xinyu;LI Min(National Engineering Research Center of Microbial Medicine,Hebei Industry Microbial Metabolic Engineering & Technology Research Center,New Drug Research and Development Co.,Ltd.,North China Pharmaceutical Corporation,Shijiazhuang 050015,China)

机构地区：[1]微生物药物国家工程研究中心河北省工业微生物代谢工程技术研究中心华北制药集团新药研究开发有限责任公司,河北石家庄050015

出　　处：《化学与生物工程》2019年第4期10-12,23,共4页Chemistry & Bioengineering

基　　金：国家"重大新药创制"科技专项(2014ZX09201001-002)

摘　　要：研究了大孔吸附树脂分离纯化棘白霉素B母核(ECBN)的工艺条件。以大孔吸附树脂D303为脱色树脂、HZ835为吸附树脂,将ECBN滤液pH值调至5.5进行吸附,吸附量为14 512μg·mL^(-1);再以5%乙醇水溶液为解吸剂解吸,解吸率为91.8%;最后经纳滤浓缩、脱炭、结晶、真空干燥等工艺得到ECBN粗粉,含量为65%左右,色谱纯度大于80%,提取总收率超过60%。该工艺简单易行、操作成本低、溶媒消耗少,适合工业化生产。We studied the separation and purification conditions of Echinocandin B nucleus (ECBN) by macroporous adsorption resin.Using resin D303 as a decolorization resin,resin HZ835 as an adsorption resin,and 5% ethanol aqueous solution as a desorbent,the ECBN (pH=5.5) is dynamically adsorbed and desorbed with an adsorption capacity of 14 512 μg·mL ^-1 and a desorption rate of 91.8%,respectively.Then,through nanofiltration concentration,decarbonization,crystallization,and vacuum drying,we obtained ECBN crude powder with the content of about 65%,the purity of more than 80%,and the total yield of more than 60%.The process has the advantages of simple operation,low cost,low solvent consumption,which is suitable for industrial production.

关 键 词：棘白霉素B母核 大孔吸附树脂 分离纯化 

分 类 号：TQ465.92[化学工程—制药化工]