不同诱导处理对番茄叶片营养物质含量及防御酶活性的影响  

Effects of different induction treatments on contents of nutrient and activities of defense enzymes in the leaves of tomato plant

作　　者：蒲恒浒 郅军锐[1] 曾广[1] 岳文波 PU Heng-Hu;ZHI Jun-Rui;ZENG Guang;YUE Wen-Bo(Guizhou Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region,Institute of Entomology,Guizhou University,Guiyang 550025,China;Forestry Department of Guizhou Province,Guiyang 550001,China)

机构地区：[1]贵州大学昆虫研究所贵州省山地农业病虫害重点实验室,贵阳550025 [2]贵州省林业厅,贵阳550001

出　　处：《环境昆虫学报》2019年第2期405-412,共8页Journal of Environmental Entomology

基　　金：国家自然科学基金(31160367);贵州省国际科技合作基地项目(黔科合平台人才[2016]5802)

摘　　要：本文研究了番茄在不同时间下受西花蓟马Frankliniella occidentalis危害(DTF)、机械损伤(MW)、茉莉酸(JA)和水杨酸甲酯(MeSA)外源诱导后,叶片营养物质含量和防御酶活性的变化。结果表明:各种诱导处理24 h和36 h时,番茄叶片可溶性蛋白和可溶性糖含量不同程度的下降,其中虫害处理36 h时,叶片可溶性蛋白和可溶性糖含量下降最明显;在48 h时除水杨酸甲酯处理外,番茄营养物质含量均显著升高。β-1,3葡聚糖酶(PR-2)活性在虫害、机械损伤和茉莉酸处理24 h和36 h后均升高,其中虫害处理的PR-2活性最高。各种处理均能诱导番茄叶片苯丙氨酸解氨酶(PAL)活性明显升高,且均随时间的延长持续升高。所有处理24 h时的番茄叶片多酚氧化酶(PPO)均被激发。各种处理均能导致植株的脂氧合酶(LOX)活性升高,但不同处理诱导的LOX活性升高的时间不同。结果表明,番茄能通过改变营养物质含量和防御酶活性对不同诱导处理作出生理应激反应,但反应程度与诱导方式和时间有关。The changes of contents of nutrient and activities of defense enzymes, in the leaves of tomato plant treated by Frankliniella occidentalis feeding (DTF), mechanical damage (MW), jasmonic acid (JA) and methyl salicylate (MeSA) induction respectively were studied at different time. The results showed that the contents of soluble protein and soluble sugar of tomato leaves subjected to different treatments decreased significantly at 24 h and 36 h compared to control, and they decreased most obviously treated by DTF at 36 h. The contents of nutrient in the leaves of tomato rose significantly at 48 h , except for plant treated by MeSA. The activities of PR-2 increased significantly subjected to DTF, MW and JA at 24 h and 36 h, and which treated by DTF were highest. The activities of PAL subjected to various treatments increased over time and rose continuously with time extension. The activities of PPO in various treatments rose at 24 h. The activities of LOX increased subjected to the all treated, and the time of activities of LOX rose were different at different treatments. The results indicated that DTF, MW, JA and MeSA could induce tomato resistance, and tomato produced response by adjusting the contents of various nutrients and activities of defense enzymes. The responses were related to treatment types and treating time.

关 键 词：西花蓟马 茉莉酸 水杨酸甲酯 番茄 营养物质 防御酶 

分 类 号：Q966[生物学—昆虫学] S433.89[农业科学—农业昆虫与害虫防治]