稀释液及解冻条件对湖羊冻精质量的影响  

Effects of diluents and thawing conditions on the quality of Hu rams semen freezing

作　　者：吕松洁 张树山[2,3] 戴建军[2,3] 孙玲伟 葛雷 梁菲 张德福[2,3] LU Song-jie;ZHANG Shu-shan;DAI Jian-jun;SUN Ling-wei;GE Lei;LIANG Fei;ZHANG De-fu(College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China;Institute ofAnimal Science and Veterinary Medicine,Shanghai Academy of Agricultural Sciences,Shanghai 201106,China;Division of Animal Genetic Engineering,Shanghai Municipal Key Laboratory of Agri-genetics and Breeding,Shanghai 201106,China;Department of Reproductive Physiology,Zhejiang Academy of Medical Sciences,Hangzhou 310013,China)

机构地区：[1]上海海洋大学水产与生命学院,上海201306 [2]上海市农业科学院畜牧兽医研究所,上海201106 [3]上海市农业遗传育种重点实验室动物遗传工程研究室,上海201106 [4]浙江省医学科学院生殖生理研究所,杭州310013

出　　处：《上海农业学报》2019年第2期63-66,共4页Acta Agriculturae Shanghai

基　　金：上海市种业发展项目[沪农科种字(2017)第3-3];上海市科委实验动物项目(17140900100);浙江省自然基金青年基金项目(LQ14C120001)

摘　　要：为优化湖羊冻精生产工艺,提高冻精质量,以湖羊种公羊的精液为试验材料,分别选择7种稀释液进行处理,采用液氮熏蒸法进行冷冻,并设置不同解冻温度和时间进行交叉试验。结果表明:7号稀释液处理的湖羊精子在42℃和70℃两种解冻条件下的活力、活率、质膜完整率和顶体完整率均最高; 6种解冻条件中,70℃解冻5 s的解冻效果最佳,其活率、活力、质膜完整性和顶体完整率分别达50. 62%、43. 09%、40. 09%和54. 37%。综上,7号稀释液和70℃解冻5 s是湖羊精液冷冻的最佳稀释液和解冻条件。In order to optimize freezing process and improve the efficiency of Hu ram semen freezing,this study froze and thawed sperms using 0.25 mL straw,liquid nitrogen fumigation method under different defrost temperature and time with seven diluents(1-7)for cross test.The results showed that No.7 diluent got the highest motility,viability,plasma integrity and acrosome integrity in both 42 ℃ and 70 ℃ thawing conditions;70 ℃ 5 s got the best result in 6 thawing conditions.The motility,viability,plasma integrity and acrosome integrity of 70 ℃ 5 s group were 50.62%,43.09%,40.09% and 54.37%,which was higher than other groups.In conclusion,No.7 diluent was the optimal freezing diluent formulation and 70 ℃ 5 s was the best thawing condition.

关 键 词：湖羊 精液冷冻 稀释液 解冻条件 

分 类 号：S826[农业科学—畜牧学]