化学生物学综合创新实验——基于pMAL蛋白融合表达系统的蛋白纯化  

Comprehensive Innovation of Chemical Biology——pMAL Protein Fusion and Purification System

作　　者：赵仲麟[1] 曹占奇 袁超[1] 吕东灿[1] 孟彦羽[1] 李瑞歌[1] ZHAO Zhonglin;CAO Zhanqi;YUAN Chao;LV Dongcan;MENG Yanyu;LI Ruige(College of Sciences,Henan Agricultural University,Henan Zhengzhou 450002 PRC)

机构地区：[1]河南农业大学理学院,河南郑州450002

出　　处：《南方农机》2019年第7期6-7,共2页

基　　金：国家自然科学基金(31100067)

摘　　要：化学生物学是当代出现的一门新兴交叉学科,其专业实验课程的建设成为很多高等院校教学改革中的重要课题。本文介绍了一个基于麦芽糖结合蛋白的蛋白质纯化的系统,使用E.coli克隆载体pMAL-p5X,适用于表达及纯化重组蛋白质。可得到无亲和标签的目的蛋白,减低其对蛋白质的影响,优于以往的蛋白质亲和层析方法。此实验可作为化学生物学专业本科或研究生综合性创新实验内容。Chemical biology is a frontier interdiscipline.The construction of its specialized experimental course has become an important subject in the teaching reform of many colleges.In this paper,a protein purification system based on maltose binding protein is introduced.The plasmid vector pMAL-p5X is an E.coli cloning vector.It is suitable for expression and purification of recombinant protein in protein fusion expression and purification system.The incompatibility label of the target protein can reduce its influence on protein,which is superior to the previous methods of protein affinity chromatography.The experiment can be used as a comprehensive innovation experiment for undergraduate or graduate students of chemical biology.

关 键 词：化学生物学 麦芽糖结合蛋白 蛋白纯化 创新实验 

分 类 号：G642[文化科学—高等教育学]