Preliminary Study on Biological Control of Mycotoxin Contamination in Agricultural Products and Its Mechanism  

作　　者：Shoumin JIA Suyan GAO Sining TANG Litian ZHANG Haitao ZHAO Yuli WANG Aili XIANG Zhaoju WU Lixue DONG 

机构地区：[1]Animal Husbandry and Veterinary Bureau of Laoting County [2]Hebei Agricultural Products Quality and Safety Testing Innovation Center [3]Tangshan Municipal Food and Drug Inspection and Testing Center

出　　处：《Agricultural Biotechnology》2019年第2期205-208,215,共5页农业生物技术（英文版）

基　　金：Supported by Forage Industry Technical System Project of Hebei Province(HBCT2018160403)

摘　　要：Aflatoxin is the most powerful carcinogen and highly toxic mycotoxin known in nature. It can cause serious toxin pollution to agricultural products, foods and fodders, and is extremely harmful to human and animal health. For aflatoxin pollution, the emphasis is on the control of source, and biological control is favored by scientists because of its advantages of environmental protection and ecological safety. According to the morphology, color and appearance of microbial colonies isolated from maize kernels, 300 bacterial strains were obtained from main maize-producing areas including Tangshan City, Handan City, Shijiazhuang City and Cangzhou City. Two biocontrol bacterial strains(B120 and B65) having a strong control effect on aflatoxins were screened. After identification by molecular biology and physical and chemical properties, the two biocontrol bacterial strains were identified as Bacillus amyloliquefaciens. Their effects on spore germination, mycelial growth and toxin degradation of Aspergillus flavus were investigated. The results showed that strains B120 and B65 could significantly inhibit the spore germination and mycelial growth of A. flavus and reduce aflatoxin production. This study provides a new way to prevent and control the pollution control of aflatoxins in agricultural products.Aflatoxin is the most powerful carcinogen and highly toxic mycotoxin known in nature. It can cause serious toxin pollution to agricultural products, foods and fodders, and is extremely harmful to human and animal health. For aflatoxin pollution, the emphasis is on the control of source, and biological control is favored by scientists because of its advantages of environmental protection and ecological safety. According to the morphology, color and appearance of microbial colonies isolated from maize kernels, 300 bacterial strains were obtained from main maize-producing areas including Tangshan City, Handan City, Shijiazhuang City and Cangzhou City. Two biocontrol bacterial strains(B120 and B65) having a strong control effect on aflatoxins were screened. After identification by molecular biology and physical and chemical properties, the two biocontrol bacterial strains were identified as Bacillus amyloliquefaciens. Their effects on spore germination, mycelial growth and toxin degradation of Aspergillus flavus were investigated. The results showed that strains B120 and B65 could significantly inhibit the spore germination and mycelial growth of A. flavus and reduce aflatoxin production. This study provides a new way to prevent and control the pollution control of aflatoxins in agricultural products.

关 键 词：Agricultural products Food FODDER AFLATOXIN CONTAMINATION Bioncontrol BACTERIA Control effect 

分 类 号：S[农业科学]