民族药四数九里香的化学成分及其细胞毒活性研究  

Compounds isolated from Murraya tetramera Huang and their cytotoxic activity

作　　者：周永福 吴明珠[2] 陈鸿平[1] 刘友平[1] ZHOU Yong-fu;WU Ming-zhu;CHEN Hong-ping;LIU You-ping(Pharmacy School of Chengdu University of TCM,Chengdu 610075,China;ChongQing Industry Polytechnic College,Chongqing 401120,China)

机构地区：[1]成都中医药大学药学院,成都611137 [2]重庆工业职业技术学院化学与制药工程学院,重庆401120

出　　处：《天然产物研究与开发》2019年第4期627-632,共6页Natural Product Research and Development

基　　金：重庆市教育委员会科学技术研究计划青年项目(KJ1603006)

摘　　要：为了阐明民族药四数九里香Murraya tetramera Huang的药效物质基础,课题组使用色谱技术从其醇提取物中分离得14个化合物;运用~1H NMR、^(13)C NMR波谱方法和文献数据对比鉴定为正24烷酸(1)、9,13,17,21-tetramethyl-5-docosenoic acid(2)、3-O-β-D-吡喃葡萄糖基-山奈酚甙(3)、补骨脂素(4)、紫苏醛(5)、槲皮素(6)、methyl salicylate glucoside(7)、(9S,10R,11E,13R,15Z)-9,10,13-trihydroxyoctadeca-11,15-dienoic Acid(8)、2-isopropyl-5-methylphenol(9)、β-胡萝卜甘(10)、7-羟基香豆素(11)、七叶内酯(12)、β-谷甾醇醋酸酯(13)、山奈酚(14)。除了化合物4、6外,其他化合物为首次从该植物中分离得到。同时,研究各化合物对5株肿瘤细胞的体外生长抑制作用。与阳性对照组比较,结果表明:化合物3、4、5、6、7、8、10、11、12、13、14对白血病HL60显现良好的细胞毒活性;化合物1～14对肺腺癌A549的的细胞毒活性低于阳性对照组;化合物6、13、14对肝癌SMMC7721显现良好的细胞毒活性;化合物1、2、3、6、7、9、11、12对乳腺癌MCF7显现良好的细胞毒活性;化合物1～14对结肠癌SW480的细胞毒活性低于阳性对照组。The compounds from Murraya tetramera Huang and their cytotoxic activities were investigated in this study.The ethanol extract from M.tetramera Huang were separated by chromatographic techniques.The structures of these compounds were identified by 1H-NMR,′ 3C-NMR.They were n-24 alkanoic acid ( 1 ),9,13,17,21-tetramethyl-5-docosenoic acid ( 2 ),3- O-β-D-glucopyranosyl-kaempferol glycoside ( 3),psoralen(4),perillaldehyde (5),quercetin (6),methyl salicylate glucoside(7 ),(9 S ,10 R ,11 E ,13 R ,15 Z )-9,10,13-trihydroxyoctadeca-11,15-dienoic acid( 8 ),2-isopropyl-5-methylphenol ( 9 ),β-sitosterol-3- O-β-D-glucopy-ranosyl ( 10 ),7-hydroxycoumarin ( 11),esculetin (12),β-sitosterol acetate (13),kaempferol (14).All of these compounds were isolated from this plant for the first time except compound (4) and (6 ).The cell inhibition rates of compounds against 5 tumor cells in vitro were studied.Compared with the positive control group,the results showed that the cell inhibition rates of compounds 3,4,5,6,7,8,10,11,12,13 and 14 showed good cytotoxicity to HL60,The cell inhibition rates of compounds 6,13 and 14 showed good cytotoxicity to SMMC7721,Compounds 1,2,3,6,7,9,11,12 showed good cytotoxicity to MCF7,and compounds 1-14 showed good cytotoxicity to A549 and MCF7.The cell inhibition rates of compounds 1-14 ,against A549 and SW480 were lower than the positive control group.

关 键 词：四数九里香 民族民间药 化学成分 细胞毒活性 核磁共振 

分 类 号：R284.2[医药卫生—中药学] R965[医药卫生—中医学]