荧光内滤效应技术在生物检测和疾病标志上的应用  

Application of Inner Filter Effect Technology in Biological Detection and Disease Markers

作　　者：田丹碧 吴胜男 张浩 江凌[2] 霍峰蔚[3] Danbi Tian;Shengnan Wu;Hao Zhang;Ling Jiang;Fengwei Huo(College of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing 211816, China;College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China;Institute of Advanced Materials, Nanjing Tech University, Nanjing 211816, China)

机构地区：[1]南京工业大学化学与分子工程学院,南京211816 [2]南京工业大学食品与轻工学院,南京211816 [3]南京工业大学先进材料研究院,南京211816

出　　处：《化学进展》2019年第2期413-421,共9页Progress in Chemistry

基　　金：国家自然科学基金联合基金项目(No.U1603112;21574065);国家杰出青年科学基金(No.21625401)资助~~

摘　　要：荧光内滤效应(inner filter effect,IFE)作为一个重要的非辐射能量转换模型,其作用机理是吸收剂的吸收光谱与荧光剂的激发光谱或发射光谱或两者同时发生谱带重叠,导致荧光剂的激发峰/发射峰的荧光被猝灭。合适的供体-受体对是成功组建IFE传感器检测目标物的重要因素。近年来,IFE技术以其操作简单、灵敏度高,无需修饰供体,无需供体与受体连接,已经引起科研工作者广泛的研究。早期诊断和早期治疗是预防疾病发生和保护健康最为有效的办法。本综述总结了近几年来IFE技术检测酶活、农药、代谢物及小分子化学物质等生物标志物在疾病标志和健康监控上的研究成果,分析了基于"turn-off"法、"turn-on"法以及"ratiometric fluorescence assay"法构建的IFE传感器的独特设计并讨论了各方法的优缺点。最后,简要指出了IFE技术在疾病标志和健康监控上的优点和实际应用时所存在的障碍,并对IFE技术和疾病监控手段未来的发展前景进行了展望。As an important non-irradiation energy conversion model, the mechanism of inner filter effect(IFE) is that the absorption spectrum of the absorbers overlaps with the excitation spectrum or emission spectrum or both spectra of the fluorophor, resulting in the fluorescence quenching of the excitation peak/emission peak of the fluorophor. The suitable donor-acceptor pair is a significant factor for successfully constructing IFE sensors to detect targets. In recent years, IFE technology has been widely studied by researchers because of its simple operation, high sensitivity, no need to modify donor and link donor and receptor. Disease is a serious threat to people's health. Early diagnosis and early treatment are the most effective ways to prevent disease and protect health. This review summarizes the research results of IFE technology in recent years for detecting enzyme activities, pesticides, metabolites and small molecular chemicals on disease markers and health monitoring, and explains the unique design of IFE sensors based on "turn-off","turn-on“and “ratiometric fluorescence assay” method. The advantages and disadvantages of each method are also discussed. Finally, the advantages and practical obstacles of IFE technology in disease labeling and health monitoring are briefly pointed out, and the prospects for the future development of IFE technology and disease surveillance methods are also prospected.

关 键 词：荧光内滤效应 疾病标志物 酶活 农药 代谢物 

分 类 号：O657.3[理学—分析化学] R730.43[理学—化学]