干细胞移植后示踪技术的研究进展  

Research Progress of Stem Cell Monitoring After Transplantation

作　　者：刘志燕 龙瀛 何晓晓[2] 周晓 LIU Zhiyan;LONG Ying;HE Xiaoxiao;ZHOU Xiao(The Affiliated Cancer Hospital of Xiangya School of Medicine,Central South University,Hunan Cancer Hospital,Changsha 410013,China)

机构地区：[1]中南大学湘雅医学院附属肿瘤医院/湖南省肿瘤医院,湖南省长沙市410013 [2]湖南大学生物学院,湖南省长沙市410082

出　　处：《组织工程与重建外科杂志》2019年第2期111-114,共4页Journal of Tissue Engineering and Reconstructive Surgery

基　　金：长沙市科技局重点研发项目(kq1706038);湖南省肿瘤医院科研平台项目;湖南省卫生计生委基金项目(B20180357);湖南省卫生计生委(B20180519);湖南省科技厅科卫联合项目(2018JJ6025);湖南省科技厅重点研发计划项目(2018SK2120);湖南省科技厅(2018SK7005)

摘　　要：干细胞具有再生和分化的能力,干细胞移植已经越来越多地应用于疾病的治疗。在干细胞移植的实验研究中,需要对干细胞进行标记,来观察和追踪其在宿主内的分布和增殖等情况。目前,干细胞标记主要分为直接标记和间接标记。直接标记即将标记试剂直接引入到干细胞中,间接标记是将报告基因整合到细胞中,继而检测基因表达产物。本文对现有的干细胞示踪技术的优缺点和研究进展进行综述。Stem cells have the ability to regenerate and differentiate,and stem cell transplantation has been increasingly used in the treatment of diseases.In the experimental study of stem cell transplantation,stem cells need to be labeled in order to observe and trace their distribution and proliferation in the host.There are two main classes of cell labeling methods for molecular imaging:the direct and indirect labeling methods.The direct labeling is the direct introduction of labeling reagents into stem cells,and the indirect labeling is the integration of reporter genes into cells,and then detection of the gene expression products.In this paper,the advantages and disadvantages of stem cell monitoring technology and its research progress were reviewed.

关 键 词：干细胞移植 干细胞示踪技术 干细胞标记 

分 类 号：Q813.1[生物学—生物工程]