硝化抑制剂对酸性红壤硝化作用及氨氧化微生物丰度和群落结构的影响  

Effects of Nitrification Inhibitors on Nitrification and Ammonia Oxidizers Abundance and Community Structure in an Acidic Red Soil

作　　者：黄琼 王庆 吴启华 李爽 黄莹 陈迪文 江永 HUANG Qiong;WANG Qing;WU Qi-hua;LI Shuang;HUANG Ying;CHEN Di-wen;JIANG Yong(Guangdong Provincial Bioengineering Institute (Guangzhou Sugarcane Industry Research Institute), Guangdong Key Laboratory of Sugarcane Improvement and Biorefinery, Guangzhou 510316;China Sugar Inspection Center, Guangzhou510316)

机构地区：[1]广东省生物工程研究所(广州甘蔗糖业研究所)广东省甘蔗遗传改良与生物炼制重点实验室,广东广州510316 [2]国家糖业质量监督检测中心,广东广州510316

出　　处：《甘蔗糖业》2019年第2期18-26,共9页Sugarcane and Canesugar

基　　金：广东省科学院实施创新驱动发展能力建设专项资金项目(2018GDASCX-0925);国家自然科学青年基金项目(41807054)

摘　　要：以一种典型酸性红壤为研究对象,在微宇宙培养条件下,研究硝化抑制剂双氰胺(DCD)和乙炔对酸性土壤硝化过程以及氨氧化微生物丰度和群落结构的影响。结果表明:硫酸铵(铵态氮肥)施加刺激了土壤硝化过程,硝化抑制剂处理显著抑制了硝化作用并且C_2H_2对硝化过程的抑制作用比DCD更强。研究还发现氨氧化细菌(Ammonia-Oxidizing Bacteria,AOB)的丰度与的浓度呈显著正相关关系,而氨氧化古菌(Ammonia-oxidizing Archaea,AOA)的丰度与的浓度呈显著负相关关系。末端限制性片段多态性(T-RFLP)分析和主坐标分析(PCo A)表明单施硫酸铵处理改变了AOB和AOA的群落结构。硝化抑制剂处理对AOA和AOB的群落结构没有显著影响。这些结果表明在高铵氮条件下AOB是土壤硝化过程的主要执行者,硝化抑制剂通过抑制AOB的丰度从而抑制硝化过程。Effects of two different nitrification inhibitors (NIs, DCD and C2H2) on nitrification process, ammonia oxidizers abundance as well as their community structure in an acidic red soil were studied by cultivation experiment. Our results showed that ammonium-fertilizer application significantly increased the net nitrification rates, but were significantly inhibited by both NIs, and the inhibitory effect of C2H2 was significantly greater than that of DCD. AOB amoA gene abundance was positively correlated with NO3 - concentration, while AOA amoA gene abundance was negatively correlated with it. Based on T-RFLP and PCoA, application of ammonium-fertilizer significantly changed the structure of the AOB and AOA community in the acidic soil. However, the application of NIs did not change the structure of ammonia oxidizers. These results indicated that AOB controlled soil nitrification process and the NIs inhibited nitrification by suppressing AOB growth in high ammonium soil.

关 键 词：酸性红壤 硝化作用 氨氧化细菌 氨氧化古菌 硝化抑制剂 

分 类 号：S566.1[农业科学—作物学]