牛樟芝固态发酵产物的降胆固醇作用  

Cholesterol lowering efficacy of Antrodia camphorata produced by solid state fermentation

作　　者：王召初 刘晓凤 衣振伟 张悦 夏奇娇 郭丹颖 王光强 艾连中 夏永军 WANG Zhaochu;LIU Xiaofeng;YI Zhenwei;ZHANG Yue;XIA Qijiao;GUODanying;WANG Guangqiang;AI Lianzhong;XIA Yongjun(School of Medical Instrument and Food Engineering, University of Shanghaifor Science and Technology,Shanghai Engineering Research Center of Food Microbiology, Shanghai,200093)

机构地区：[1]上海理工大学医疗器械与食品学院上海食品微生物工程技术研究中心,上海200093

出　　处：《工业微生物》2019年第2期12-18,共7页Industrial Microbiology

基　　金：国家自然科学基金面上项目(31871757);上海市大学生创新创业训练计划项目(XJ10252258);沪江基金研究基地专项(D15012)

摘　　要：本实验主要探究牛樟芝(S-29)固态发酵产物对高脂饮食小鼠胆固醇调节的影响。小鼠随机分为正常组、高脂模型组、护肝片阳性对照组、固态发酵组及液态发酵组;小鼠经高脂饲料喂养6周,相应物质灌胃4周。检测小鼠血清及肝脏相关指标; q-PCR检测胆固醇代谢相关基因的mRNA表达量。结果表明,与模型组比较,固态发酵组小鼠血清游离脂肪酸(NEFA)及谷丙转氨酶(ALT)浓度显著降低,分别降低了38. 5%和40. 7%;肝脏总胆固醇(TC)浓度显著降低,降低了23. 5%;低密度脂蛋白受体(LDL-R)的mRNA表达量显著增加,增加了3. 6倍。结果证明,牛樟芝固态发酵产物具有较好的降胆固醇作用,其主要机制可能是通过上调LDL-R基因的表达,以促进胆固醇的分解代谢,进而降低小鼠体内胆固醇浓度。The cholesterol regulation efficacy of Antrodia camphorata (S- 29) produced by solid state fermentation (SSF) in mice with high fat diet was studied. The mice were randomly divided into normal group, high fat model group, liver-protecting tablet positive control group, solid-state fermentation group and submerged fermentation group. The mice were fed with high fat diet for 6 weeks, and the corresponding substances were given for 4 weeks. The serum and liver relevant indexes of the mice were measured, and the mRNA expression levels of genes related to cholesterol metabolism were analyzed by q-PCR. Comparing to those in the model group, the NEFA and ALT concentrations of serum in the solid-state fermentation group were significantly decreased by 38.5% and 40.7%, respectively;the liver TC concentration was significantly decreased by 23.5%;and the mRNA expression level of LDL-R was significantly increased by 3.6 times. The above results suggested that the solid fermentation products of Antrodia camphorata had good cholesterol-lowering effects, and its main mechanism might be to up-regulate the expression of LDL-R gene in order to promote cholesterol catabolism and then reduce cholesterol concentration in mice.

关 键 词：牛樟芝 固态发酵 降胆固醇 LDL-R 

分 类 号：R965[医药卫生—药理学]