液基薄层细胞学技术联合免疫荧光染色在尿足细胞检测中的应用  

Application of thinprep liquid-based cytologic test combined with immunofluorescence staining in the detection of urinary podocytes

作　　者：孙新平 戴菊华 张捷[2] SUN Xinping;DAI Juhua;ZHANG Jie(Department of Clinical Laboratory, Peking University International Hospital, Beijing 102206;Department of Clinical Laboratory, Peking University Third Hospital, Beijing 100191, China)

机构地区：[1]北京大学国际医院检验科,北京102206 [2]北京大学第三医院检验科,北京100191

出　　处：《临床检验杂志》2019年第3期173-176,共4页Chinese Journal of Clinical Laboratory Science

基　　金：北京大学国际医院中青年启动项目(YN2016QN12)

摘　　要：目的观察液基薄层细胞学技术(TCT)联合间接免疫荧光染色对尿液中足细胞的染色效果并探讨其临床应用价值。方法收集50例2型糖尿病肾病患者和14例健康对照者晨尿标本,TCT制片与传统离心涂片后均行间接免疫荧光染色,观察足细胞形态。结果 64例标本中TCT制片满意率(85.94%)高于传统离心涂片(50.00%),TCT制片的足细胞检出率(73.47%)高于传统离心涂片(51.02%),差异有统计学意义(P<0.05)。2种方法检测足细胞均阳性时,TCT制片的阅片效果优于传统离心涂片。结论较传统离心涂片而言,TCT制片联合间接免疫荧光染色在尿足细胞检测中具有优越性。Objective To compare the indirect immunofluorescence staining effect of urinary podocytes by thinprep liquid-based cytologic test (TCT) with that by the conventional centrifugal smear method, and explore its clinical application value. Methods The morning urine samples from 50 patients with type 2 diabetic nephropathy and 14 healthy controls were smeared with the TCT and conventional centrifugation method, respectively, and then the indirect immunofluorescence staining were performed to observe the morphology of podocytes. Results For 64 urine samples, the satisfactory rate of TCT smears (85.94%) was significantly higher than that of conventional smears (50.00%), and the podocyte detection rate of TCT smears (73.47%) was also significantly higher than that of conventional smears (51.02%)( P <0.05). When urinary podocytes of the same patient were positive by both methods, the reading effect of TCT smears was obviously superior to that of conventional smears. Conclusion The TCT combined with indirect immunofluorescence staining is obviously superior to the conventional centrifugal smear method in the podocyte diagnosis of urine samples.

关 键 词：液基薄层细胞学技术 传统离心涂片 间接免疫荧光染色 足细胞 

分 类 号：R446[医药卫生—诊断学]