卟啉多孔化合物的合成及其在光电免疫传感器中的应用  

Synthesis of Porous Porphyrin Compound and Its Application in Photoelectric Immunosensor

作　　者：王敏 孟爽 谷梦巧 迟宽能 马玉婵 邓燕 李德蕾 张茜[1] 胡蓉[1] 杨云慧[1] WANG Min;MENG Shuang;GU Meng-qiao;CHI Kuan-neng;MA Yu-chan;DENG Yan;LI De-lei;ZHANG Xi;HU Rong;YANG Yun-hui(College of Chemistry and Chemical Engineering,Yunnan Normal University,Kunming 650500,China)

机构地区：[1]云南师范大学化学化工学院,云南昆明650500

出　　处：《分析测试学报》2019年第3期283-288,共6页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(21465026;21765026)

摘　　要：构建了以卟啉多孔化合物-金纳米颗粒(Au NPs@PAF)为固定基质的无标记型C-反应蛋白(CRP)光电免疫传感器。在最优条件下,采用计时电流法实现了对CRP的定量检测。该传感器在CRP质量浓度0.05～60 ng/mL范围内与光电流有较好的线性关系,检出限为0.017 ng/mL,相关系数为0.994 6,平均回收率为102%,具有良好的选择性,为C-反应蛋白的检测提供了一种灵敏的方法。In this paper,a label-free C-reactive protein(CRP)photoelectricimmunosensor was constructed using porous porphyrin compound-Au nanoparticles(Au NPs@PAF)as an immobilized substrate.Under the optimal conditions,the CRP was quantitatively detected by chronograph current method.There was a good linear relationship for the sensor versus photocurrent in the range of 0.05-60 ng/mL,with a linear correlation coefficient of 0.994 6.The limit of detection was 0.017 ng/mL,and the average recovery was 102%.This sensor provided a sensitive method for the detection of C-reactive protein with good selectivity.

关 键 词：卟啉多孔材料 光电免疫传感器 C-反应蛋白 应用 

分 类 号：O657.3[理学—分析化学]