纸芯片显色法用于L-半胱氨酸的快速测定  

Rapid Detection of L-Cysteine by Colorimetric Method Based on Paper-based Microfluidic Chip

作　　者：张剑 谢婷婷[1] 杨玉莹 刘春叶[1] Frank A.GOMEZ Wilson LEE 肖倩 ZHANG Jian;XIE Ting-ting;YANG Yu-ying;LIU Chun-ye;Wilson LEE(School of Pharmacy,Xi an Medical University,Xi'an 710021,China;Department of Chemistry and Biochemistry,California State University,Los Angeles 90001,USA)

机构地区：[1]西安医学院药学院,陕西西安710021 [2]加州州立大学化学与生物化学系,美国洛杉矶90001

出　　处：《分析测试学报》2019年第4期477-481,共5页Journal of Instrumental Analysis

基　　金：国家自然科学基金项目(81202492);陕西省教育厅项目(18JK0670);西安医学院国家基金培育项目(2017GJFY06);西安医学院振兴人才项目(2018XNRC03);西安医学院博士科研启动基金(2018DOC17)

摘　　要：该文以5,5'-二硫硝基苯甲酸(DTNB)为含巯基化合物的衍生化试剂,建立了其纸芯片快速检测新方法。显色剂DTNB和样品在显色区相遇并显色,用手机拍照记录,采用Photoshop软件分析显色强度,进行比色检测,并以L-半胱氨酸(L-Cys)为例,优化得到纸芯片检测L-Cys的最佳显色条件。结果显示,L-Cys浓度在0.01～0.1 mmol/L范围内与显色强度呈良好的线性关系(r=0.999 8),检出限达0.001 mmol/L。多种氨基酸和干扰离子对L-Cys在纸芯片上的测定干扰不超过±5%。采用该方法测得牛血清样品中L-Cys含量为0.013 mmol/L,加标回收率为99.1%～103%,该方法检测L-Cys具有操作简单、耗样量少、检测快速、重现性好等优点。A new colorimetric method based on paper chip was established for the rapid detection of L -Cysteine ( L -Cys ) using 5 ,5 -dithiobis-(2-nitrobenzoic acid )(DTNB ) as derivatizing agent.A normal phone was used to record the color intensity.The color intensity was analyzed by Photoshop software and used to calculate the concentration of thiol-contained molecules in samples.The optimum coloration conditions for the detection were investigated.Results showed that there was a good linear relationship between color intensity and L -Cys concentration in range of 0.01-0.1 mmol/L with the linear correlation coefficient of 0.999 8 and the detection limit of 0.001 mmol/L.In the range of ±5 %,the interference of several amino acids and ions on the determination of L -Cyse was small.The method was applied in the detection of L -Cys content in bovine serum sample with a result of 0.013 mmol/L and a spiked recovery of 99.1 %-103 %.Results showed that this method has high selectivity and reproducibility.The colorimetric method based on paper-based microfluidic chip has several advantages such as simplicity,low-reagent consumption,rapidness and repeatability.

关 键 词：纸芯片 巯基化合物 5 5-二硫硝基苯甲酸(DTNB) 比色法 

分 类 号：O656.3[理学—分析化学]